Assignment of 35 single-copy and 17 repetitive sequence DNA probes to human chromosome 3: High-resolution physical mapping of 7 DNA probes by in situ hybridization

Lakshmi Atchison, Linda Cannizzaro, Jorge Caamano, Michael Atchison, Robert L. Comis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thirty-five single-copy and 17 repetitive sequence DNA probes specific for human chromosome 3 were isolated from human chromosome 3-derived genomic libraries. Seven DNA clones, including three that are polymorphic for BglII or MspI, were mapped by in situ hybridization. Four probes were mapped to 3p subregions and 3 were mapped to 3q subregions. Three of the DNA sequences map to regions overlapping a segment of chromosome 3 (3p14-23) frequently deleted in small cell lung cancer cells. By Southern blot analysis on a deletion hybrid panel, we previously mapped 6 of these probes to three distinct chromosome 3 subregions. Our in situ data support these assignments and more precisely determine the localization of each clone to the following regions: D3S34 (3p14-21), D3S35 (3p21), D3S39 (3p21), D3S40 (3p12-13), D3S37 (3q21-23), and D3S36 (3q21). Clone pL84c, a low repeat sequence clone (∼30 copies), was mapped to the 3q21-29 subregion. These DNA clones mapped by in situ hybridization can provide useful landmarks for the ordering and localization of other clones.

Original languageEnglish (US)
Pages (from-to)441-450
Number of pages10
JournalGenomics
Volume6
Issue number3
DOIs
StatePublished - 1990
Externally publishedYes

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Chromosomes, Human, Pair 3
Nucleic Acid Repetitive Sequences
DNA Probes
Human Chromosomes
In Situ Hybridization
Clone Cells
Genomic Library
DNA
Small Cell Lung Carcinoma
Southern Blotting

ASJC Scopus subject areas

  • Genetics

Cite this

Assignment of 35 single-copy and 17 repetitive sequence DNA probes to human chromosome 3 : High-resolution physical mapping of 7 DNA probes by in situ hybridization. / Atchison, Lakshmi; Cannizzaro, Linda; Caamano, Jorge; Atchison, Michael; Comis, Robert L.

In: Genomics, Vol. 6, No. 3, 1990, p. 441-450.

Research output: Contribution to journalArticle

Atchison, Lakshmi ; Cannizzaro, Linda ; Caamano, Jorge ; Atchison, Michael ; Comis, Robert L. / Assignment of 35 single-copy and 17 repetitive sequence DNA probes to human chromosome 3 : High-resolution physical mapping of 7 DNA probes by in situ hybridization. In: Genomics. 1990 ; Vol. 6, No. 3. pp. 441-450.
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