TY - JOUR
T1 - Assignment of 35 single-copy and 17 repetitive sequence DNA probes to human chromosome 3
T2 - High-resolution physical mapping of 7 DNA probes by in situ hybridization
AU - Atchison, Lakshmi
AU - Cannizzaro, Linda
AU - Caamano, Jorge
AU - Atchison, Michael
AU - Comis, Robert L.
N1 - Funding Information:
The authors are very thankful to Ms. Lisa Carosiello for skillful and diligent typing of this manuscript. Research was supported by National Institute of Health Grant RR05895, by a Biomedical Research Support Grant awarded to Fox Chase Cancer Center from Institutional funds, and by the Commonwealth of Pennsylvania.
PY - 1990/3
Y1 - 1990/3
N2 - Thirty-five single-copy and 17 repetitive sequence DNA probes specific for human chromosome 3 were isolated from human chromosome 3-derived genomic libraries. Seven DNA clones, including three that are polymorphic for BglII or MspI, were mapped by in situ hybridization. Four probes were mapped to 3p subregions and 3 were mapped to 3q subregions. Three of the DNA sequences map to regions overlapping a segment of chromosome 3 (3p14-23) frequently deleted in small cell lung cancer cells. By Southern blot analysis on a deletion hybrid panel, we previously mapped 6 of these probes to three distinct chromosome 3 subregions. Our in situ data support these assignments and more precisely determine the localization of each clone to the following regions: D3S34 (3p14-21), D3S35 (3p21), D3S39 (3p21), D3S40 (3p12-13), D3S37 (3q21-23), and D3S36 (3q21). Clone pL84c, a low repeat sequence clone (∼30 copies), was mapped to the 3q21-29 subregion. These DNA clones mapped by in situ hybridization can provide useful landmarks for the ordering and localization of other clones.
AB - Thirty-five single-copy and 17 repetitive sequence DNA probes specific for human chromosome 3 were isolated from human chromosome 3-derived genomic libraries. Seven DNA clones, including three that are polymorphic for BglII or MspI, were mapped by in situ hybridization. Four probes were mapped to 3p subregions and 3 were mapped to 3q subregions. Three of the DNA sequences map to regions overlapping a segment of chromosome 3 (3p14-23) frequently deleted in small cell lung cancer cells. By Southern blot analysis on a deletion hybrid panel, we previously mapped 6 of these probes to three distinct chromosome 3 subregions. Our in situ data support these assignments and more precisely determine the localization of each clone to the following regions: D3S34 (3p14-21), D3S35 (3p21), D3S39 (3p21), D3S40 (3p12-13), D3S37 (3q21-23), and D3S36 (3q21). Clone pL84c, a low repeat sequence clone (∼30 copies), was mapped to the 3q21-29 subregion. These DNA clones mapped by in situ hybridization can provide useful landmarks for the ordering and localization of other clones.
UR - http://www.scopus.com/inward/record.url?scp=0025328475&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025328475&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(90)90474-9
DO - 10.1016/0888-7543(90)90474-9
M3 - Article
C2 - 2328989
AN - SCOPUS:0025328475
SN - 0888-7543
VL - 6
SP - 441
EP - 450
JO - Genomics
JF - Genomics
IS - 3
ER -