Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women

Andrea L. Niklaus, Mira Aubuchon, Gregory Zapantis, Ping Li, Hong Qian, Barbara Isaac, Mimi Kim, Goli Adel, Jeffrey W. Pollard, Nanette F. Santoro

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: We determined protein and mRNA expressions of markers of normal human endometrial proliferation and hypothesized that dysregulation of the endometrial response to estradiol (E2) and progesterone would be observed in the older menopausal transition (MT) women compared with mid-reproductive age (MRA) controls. Methods: Endometrial biopsies were prospectively obtained from MRA and MT non-randomized healthy volunteers during proliferative (± exogenous E2) and secretory (MRA only) menstrual cycle phases. mRNA and/or nuclear protein expressions of proliferative markers (MKI67, PCNA and MCM2), cell-cycle regulators (cyclins A1, E1 and D1 and cyclin dependant kinase Inhibitor B; CCNA1, CCNE1, CCND1 and CDKN1B) and sex-steroid receptors [estrogen receptor (ER) and progesterone receptor (PR)] were assessed in endometrial lumen, gland and stroma. Results: MRA women had significantly higher proliferative than secretory expression of MKI67, PCNA, MCM2, CCNA1, CCNE1, ESR1 and PGR in lumen and gland (minimal stromal changes), whereas CDKN1B protein expression was higher during the secretory phase. E2-treatment of MT women led to relatively less MKI67 glandular protein expression compared with MRA women; no other age-related differences were observed. Conclusion: Although the MT does not appear to alter the proliferative cell phenotype of endometrial epithelium and stroma, the data suggest that prior to the MT, age is associated with a decrease in some proliferative markers and steroid receptor expression status within different endometrial cell types.

Original languageEnglish (US)
Pages (from-to)1778-1788
Number of pages11
JournalHuman Reproduction
Volume22
Issue number6
DOIs
StatePublished - Jun 2007

Fingerprint

Endometrium
Epithelial Cells
Steroid Receptors
Proliferating Cell Nuclear Antigen
Cyclin A1
Cyclin-Dependent Kinase Inhibitor p27
Messenger RNA
Cyclins
Cyclin D1
Progesterone Receptors
Menstrual Cycle
Nuclear Proteins
Estrogen Receptors
Progesterone
Estradiol
Healthy Volunteers
Cell Cycle
Proteins
Phosphotransferases
Epithelium

Keywords

  • Cell cycle
  • Endometrium
  • Laser-capture
  • Menopausal transition
  • Proliferation

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Niklaus, A. L., Aubuchon, M., Zapantis, G., Li, P., Qian, H., Isaac, B., ... Santoro, N. F. (2007). Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. Human Reproduction, 22(6), 1778-1788. https://doi.org/10.1093/humrep/dem032

Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. / Niklaus, Andrea L.; Aubuchon, Mira; Zapantis, Gregory; Li, Ping; Qian, Hong; Isaac, Barbara; Kim, Mimi; Adel, Goli; Pollard, Jeffrey W.; Santoro, Nanette F.

In: Human Reproduction, Vol. 22, No. 6, 06.2007, p. 1778-1788.

Research output: Contribution to journalArticle

Niklaus, AL, Aubuchon, M, Zapantis, G, Li, P, Qian, H, Isaac, B, Kim, M, Adel, G, Pollard, JW & Santoro, NF 2007, 'Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women', Human Reproduction, vol. 22, no. 6, pp. 1778-1788. https://doi.org/10.1093/humrep/dem032
Niklaus, Andrea L. ; Aubuchon, Mira ; Zapantis, Gregory ; Li, Ping ; Qian, Hong ; Isaac, Barbara ; Kim, Mimi ; Adel, Goli ; Pollard, Jeffrey W. ; Santoro, Nanette F. / Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women. In: Human Reproduction. 2007 ; Vol. 22, No. 6. pp. 1778-1788.
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AU - Qian, Hong

AU - Isaac, Barbara

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AU - Pollard, Jeffrey W.

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