Assessment of copper, iron, zinc and manganese status and speciation in patients with Parkinson's disease: A pilot study

Olga P. Ajsuvakova, Alexey A. Tinkov, Desiree Willkommen, Anastasia A. Skalnaya, Alexey B. Danilov, Anna A. Pilipovich, Michael Aschner, Anatoly V. Skalny, Bernhard Michalke, Margarita G. Skalnaya

Research output: Contribution to journalArticle

Abstract

Background: The objective of this pilot study was to assess iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) status (hair, serum, and urine) and speciation (serum) in Parkinson's disease (PD) patients. Methods: A pilot study involving a total of 27 subjects (13 PD patients, 14 controls) was performed. Serum, urine, and hair metal content was assessed using ICP-MS. Speciation analysis of Cu, Zn, Fe, and Mn was performed using a hybrid HPLC-ICP-MS system. Results: Group comparisons did not reveal any significant group difference in serum Cu, Zn, Fe, and Mn total metal level between PD patients and controls. Speciation analysis revealed a significant decrease in Cu/ceruloplasmin copper in association with elevation of low-molecular weight species (amino acids)-bound copper. It is proposed that in PD, binding of Cu(II) ions to ceruloplasmin is reduced and free copper ions coordinate with low molecular weight ligands. The level of Mn-albumin complexes in PD patients was more than 4-fold higher as compared to the respective value in the control group. The observed difference may be considered as a marker of redistribution between high and low molecular weight ligands. Conclusions: Metal speciation is significantly affected in serum of PD-patients. These findings are indicative of the potential role of metal metabolism and PD pathogenesis, although the exact mechanisms of such associations require further detailed studies.

Original languageEnglish (US)
Article number126423
JournalJournal of Trace Elements in Medicine and Biology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Manganese
Parkinson Disease
Zinc
Copper
Iron
Metals
Serum
Ceruloplasmin
Molecular Weight
Molecular weight
Hair
Urine
Ions
Ligands
Albumins
Metabolism
High Pressure Liquid Chromatography
Amino Acids
Control Groups

Keywords

  • Copper
  • Iron
  • Manganese
  • Parkinson’ disease
  • Speciation analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Inorganic Chemistry

Cite this

Ajsuvakova, O. P., Tinkov, A. A., Willkommen, D., Skalnaya, A. A., Danilov, A. B., Pilipovich, A. A., ... Skalnaya, M. G. (Accepted/In press). Assessment of copper, iron, zinc and manganese status and speciation in patients with Parkinson's disease: A pilot study. Journal of Trace Elements in Medicine and Biology, [126423]. https://doi.org/10.1016/j.jtemb.2019.126423

Assessment of copper, iron, zinc and manganese status and speciation in patients with Parkinson's disease : A pilot study. / Ajsuvakova, Olga P.; Tinkov, Alexey A.; Willkommen, Desiree; Skalnaya, Anastasia A.; Danilov, Alexey B.; Pilipovich, Anna A.; Aschner, Michael; Skalny, Anatoly V.; Michalke, Bernhard; Skalnaya, Margarita G.

In: Journal of Trace Elements in Medicine and Biology, 01.01.2019.

Research output: Contribution to journalArticle

Ajsuvakova, Olga P. ; Tinkov, Alexey A. ; Willkommen, Desiree ; Skalnaya, Anastasia A. ; Danilov, Alexey B. ; Pilipovich, Anna A. ; Aschner, Michael ; Skalny, Anatoly V. ; Michalke, Bernhard ; Skalnaya, Margarita G. / Assessment of copper, iron, zinc and manganese status and speciation in patients with Parkinson's disease : A pilot study. In: Journal of Trace Elements in Medicine and Biology. 2019.
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AU - Ajsuvakova, Olga P.

AU - Tinkov, Alexey A.

AU - Willkommen, Desiree

AU - Skalnaya, Anastasia A.

AU - Danilov, Alexey B.

AU - Pilipovich, Anna A.

AU - Aschner, Michael

AU - Skalny, Anatoly V.

AU - Michalke, Bernhard

AU - Skalnaya, Margarita G.

PY - 2019/1/1

Y1 - 2019/1/1

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AB - Background: The objective of this pilot study was to assess iron (Fe), copper (Cu), zinc (Zn), and manganese (Mn) status (hair, serum, and urine) and speciation (serum) in Parkinson's disease (PD) patients. Methods: A pilot study involving a total of 27 subjects (13 PD patients, 14 controls) was performed. Serum, urine, and hair metal content was assessed using ICP-MS. Speciation analysis of Cu, Zn, Fe, and Mn was performed using a hybrid HPLC-ICP-MS system. Results: Group comparisons did not reveal any significant group difference in serum Cu, Zn, Fe, and Mn total metal level between PD patients and controls. Speciation analysis revealed a significant decrease in Cu/ceruloplasmin copper in association with elevation of low-molecular weight species (amino acids)-bound copper. It is proposed that in PD, binding of Cu(II) ions to ceruloplasmin is reduced and free copper ions coordinate with low molecular weight ligands. The level of Mn-albumin complexes in PD patients was more than 4-fold higher as compared to the respective value in the control group. The observed difference may be considered as a marker of redistribution between high and low molecular weight ligands. Conclusions: Metal speciation is significantly affected in serum of PD-patients. These findings are indicative of the potential role of metal metabolism and PD pathogenesis, although the exact mechanisms of such associations require further detailed studies.

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