Assays for monitoring macroautophagy activity in T cells

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specifi c recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantifi cation of macroautophagy activity.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-153
Number of pages11
Volume1343
DOIs
StatePublished - 2015
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1343
ISSN (Print)10643745

Keywords

  • LC3
  • Lysosome
  • Macroautophagy
  • T cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Botbol, Y. M., & Macian-Juan, F. (2015). Assays for monitoring macroautophagy activity in T cells. In Methods in Molecular Biology (Vol. 1343, pp. 143-153). (Methods in Molecular Biology; Vol. 1343). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-2963-4_12