Abstract
Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specifi c recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantifi cation of macroautophagy activity.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 143-153 |
| Number of pages | 11 |
| Volume | 1343 |
| DOIs | |
| State | Published - 2015 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1343 |
| ISSN (Print) | 10643745 |
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Keywords
- LC3
- Lysosome
- Macroautophagy
- T cell
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Assays for monitoring macroautophagy activity in T cells. / Botbol, Yair M.; Macian-Juan, Fernando.
Methods in Molecular Biology. Vol. 1343 Humana Press Inc., 2015. p. 143-153 (Methods in Molecular Biology; Vol. 1343).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Assays for monitoring macroautophagy activity in T cells
AU - Botbol, Yair M.
AU - Macian-Juan, Fernando
PY - 2015
Y1 - 2015
N2 - Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specifi c recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantifi cation of macroautophagy activity.
AB - Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specifi c recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantifi cation of macroautophagy activity.
KW - LC3
KW - Lysosome
KW - Macroautophagy
KW - T cell
UR - http://www.scopus.com/inward/record.url?scp=84942901698&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84942901698&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2963-4_12
DO - 10.1007/978-1-4939-2963-4_12
M3 - Chapter
C2 - 26420715
AN - SCOPUS:84942901698
VL - 1343
T3 - Methods in Molecular Biology
SP - 143
EP - 153
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -