Aspects of signal transduction in stimulus exocytosis-coupling in Paramecium.

Birgit H. Satir, G. Busch, A. Vuoso, T. J. Murtaugh

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

This paper deals with the detailed mechanisms of signal transduction that lead to exocytosis during regulative secretion induced by specific secretagogues in a eukaryotic cell, Paramecium tetraurelia. There are at least three cellular compartments involved in the process: I) the plasma membrane, which contains secretagogue receptors and other transmembrane proteins, II) the cytoplasms, particularly in the region between the cell and secretory vesicle membranes, where molecules may influence interactions of the membranes, and III) the secretory vesicle itself. The ciliated protozoan Paramecium tetraurelia is very well suited for the study of signal transduction events associated with exocytosis because this eukaryotic cell contains thousands of docked secretory vesicles (trichocysts) below the cell membrane which can be induced to release synchronously when triggered with secretagogue. This ensures a high signal-to-noise ratio for events associated with this process. Upon release the trichocyst membrane fuses with the cell membrane and the trichocyst content undergoes a Ca2+-dependent irreversible expansion. Secretory mutants are available which are blocked at different points in the signal transduction pathway. Aspects of the three components mentioned above that will be discussed here include a) the properties of the vesicle content, its pH, and its membrane; b) the role of phosphorylation/dephosphorylation of a cytosolic 63-kilodalton (kDa)Mr protein in membrane fusion; and c) how influx of extracellular Ca2+ required for exocytosis may take place via exocytic Ca2+ channels which may be associated with specific membrane microdomains (fusion rosettes).

Original languageEnglish (US)
Pages (from-to)429-443
Number of pages15
JournalJournal of Cellular Biochemistry
Volume36
Issue number4
StatePublished - Apr 1988

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Paramecium
Signal transduction
Exocytosis
Signal Transduction
Secretory Vesicles
Paramecium tetraurelia
Membranes
Cell Membrane
Eukaryotic Cells
Cell membranes
Membrane Fusion Proteins
Membrane Microdomains
Membrane Fusion
Signal-To-Noise Ratio
Fusion reactions
Cytoplasm
Phosphorylation
Electric fuses
Signal to noise ratio
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Aspects of signal transduction in stimulus exocytosis-coupling in Paramecium. / Satir, Birgit H.; Busch, G.; Vuoso, A.; Murtaugh, T. J.

In: Journal of Cellular Biochemistry, Vol. 36, No. 4, 04.1988, p. 429-443.

Research output: Contribution to journalArticle

Satir, Birgit H. ; Busch, G. ; Vuoso, A. ; Murtaugh, T. J. / Aspects of signal transduction in stimulus exocytosis-coupling in Paramecium. In: Journal of Cellular Biochemistry. 1988 ; Vol. 36, No. 4. pp. 429-443.
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