Aspartic proteases from the nematode Caenorhabditis elegans

Structural organization and developmental and cell-specific expression of asp-1

Irina Tcherepanova, Lokesh Bhattacharyya, Charles S. Rubin, Jonathan H. Freedman

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A Caenorhabditis elegans gene (asp-1) and cDNA that encode a homologue of cathepsin D aspartic protease were cloned and characterized. The asp-1 mRNA is transcribed from a single exon, and it begins with the SL1 trans-splice leader sequence. The protein (ASP-1) is expressed as a 396-amino acid, 42.7-kDa pre-pro-peptide that is post-translationally processed into a ~40-kDa lysosomal protein. ASP-1 shares ~60% sequence identity with the aspartic protease precursor from the nematode Strongyloides stercoralis. The amino acid sequences adjacent to the two active site aspartic acid residues in ASP-1 are 100% identical to those in other eukaryotic aspartic proteases. In addition, ASP-1 contains conserved, potential disulfide bond-forming cysteine residues and N-glycosylation sites. The asp-1 gene is exclusively transcribed in the intestinal cells, with the highest levels of expression observed at late embryonic and early larval stages of development, asp-1 transcription is not observed in adult nematodes or mature larvae. Furthermore, transcription predominantly occurs in eight anterior cells of the intestine (int6-int8). Analyses of ASP-1 nucleotide and amino acid sequences revealed the presence of five additional C. elegans aspartic proteases.

Original languageEnglish (US)
Pages (from-to)26359-26369
Number of pages11
JournalJournal of Biological Chemistry
Volume275
Issue number34
DOIs
StatePublished - Aug 25 2000

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Caenorhabditis elegans
Peptide Hydrolases
Transcription
Amino Acids
Amino Acid Sequence
Genes
Strongyloides stercoralis
Glycosylation
Cathepsin D
Aspartic Acid
Disulfides
Intestines
Cysteine
Larva
Exons
Catalytic Domain
Nucleotides
Complementary DNA
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Aspartic proteases from the nematode Caenorhabditis elegans : Structural organization and developmental and cell-specific expression of asp-1. / Tcherepanova, Irina; Bhattacharyya, Lokesh; Rubin, Charles S.; Freedman, Jonathan H.

In: Journal of Biological Chemistry, Vol. 275, No. 34, 25.08.2000, p. 26359-26369.

Research output: Contribution to journalArticle

Tcherepanova, Irina ; Bhattacharyya, Lokesh ; Rubin, Charles S. ; Freedman, Jonathan H. / Aspartic proteases from the nematode Caenorhabditis elegans : Structural organization and developmental and cell-specific expression of asp-1. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 34. pp. 26359-26369.
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