Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation?

Feng Zhao, Marianne Ilbert, Ranjani Varadan, Claudia M. Cremers, Beatrice Hoyos, Rebeca Acin-Perez, Valerie Vinogradov, David Cowburn, Ursula Jakob, Ulrich Hammerling

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Protein kinase C (PKC) is activated by lipid second messengers or redox action, raising the question whether these activation modes involve the same or alternate mechanisms. Here we show that both lipid activators and oxidation target the zinc-finger domains of PKC, suggesting a unifying activation mechanism. We found that lipid agonist-binding or redox action leads to zinc release and disassembly of zinc fingers, thus triggering large-scale unfolding that underlies conversion to the active enzyme. These results suggest that PKC zinc fingers, originally considered purely structural devices, are in fact redox-sensitive flexible hinges, whose conformation is controlled both by redox conditions and lipid agonists.

Original languageEnglish (US)
Pages (from-to)757-766
Number of pages10
JournalAntioxidants and Redox Signaling
Volume14
Issue number5
DOIs
StatePublished - Mar 1 2011

Fingerprint

Zinc Fingers
Protein Kinase C
Oxidation-Reduction
Zinc
Chemical activation
Lipids
Second Messenger Systems
Hinges
Conformations
Equipment and Supplies
Oxidation
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Zhao, F., Ilbert, M., Varadan, R., Cremers, C. M., Hoyos, B., Acin-Perez, R., ... Hammerling, U. (2011). Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation? Antioxidants and Redox Signaling, 14(5), 757-766. https://doi.org/10.1089/ars.2010.3773

Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation? / Zhao, Feng; Ilbert, Marianne; Varadan, Ranjani; Cremers, Claudia M.; Hoyos, Beatrice; Acin-Perez, Rebeca; Vinogradov, Valerie; Cowburn, David; Jakob, Ursula; Hammerling, Ulrich.

In: Antioxidants and Redox Signaling, Vol. 14, No. 5, 01.03.2011, p. 757-766.

Research output: Contribution to journalArticle

Zhao, F, Ilbert, M, Varadan, R, Cremers, CM, Hoyos, B, Acin-Perez, R, Vinogradov, V, Cowburn, D, Jakob, U & Hammerling, U 2011, 'Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation?', Antioxidants and Redox Signaling, vol. 14, no. 5, pp. 757-766. https://doi.org/10.1089/ars.2010.3773
Zhao, Feng ; Ilbert, Marianne ; Varadan, Ranjani ; Cremers, Claudia M. ; Hoyos, Beatrice ; Acin-Perez, Rebeca ; Vinogradov, Valerie ; Cowburn, David ; Jakob, Ursula ; Hammerling, Ulrich. / Are zinc-finger domains of protein kinase C dynamic structures that unfold by lipid or redox activation?. In: Antioxidants and Redox Signaling. 2011 ; Vol. 14, No. 5. pp. 757-766.
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