Abstract
In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by transcription factor LHX2 plays a critical role in HF-SC behavior. Using genome-wide chromatin and transcriptional profiling of HF-SCs in vivo, we show that LHX2 directly transactivates genes that orchestrate cytoskeletal dynamics and adhesion. Conditional ablation of LHX2 results in gross cellular disorganization and HF-SC polarization within the niche. LHX2 loss leads to a failure to maintain HF-SC quiescence and hair anchoring, as well as progressive transformation of the niche into a sebaceous gland. These findings suggest that niche organization underlies the requirement for LHX2 in hair follicle structure and function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 314-327 |
| Number of pages | 14 |
| Journal | Cell Stem Cell |
| Volume | 13 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 5 2013 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Medicine
- Genetics
Cite this
Architectural niche organization by LHX2 is linked to hair follicle stem cell function. / Folgueras, Alicia R.; Guo, Xingyi; Pasolli, H. Amalia; Stokes, Nicole; Polak, Lisa; Zheng, Deyou; Fuchs, Elaine.
In: Cell Stem Cell, Vol. 13, No. 3, 05.09.2013, p. 314-327.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Architectural niche organization by LHX2 is linked to hair follicle stem cell function
AU - Folgueras, Alicia R.
AU - Guo, Xingyi
AU - Pasolli, H. Amalia
AU - Stokes, Nicole
AU - Polak, Lisa
AU - Zheng, Deyou
AU - Fuchs, Elaine
PY - 2013/9/5
Y1 - 2013/9/5
N2 - In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by transcription factor LHX2 plays a critical role in HF-SC behavior. Using genome-wide chromatin and transcriptional profiling of HF-SCs in vivo, we show that LHX2 directly transactivates genes that orchestrate cytoskeletal dynamics and adhesion. Conditional ablation of LHX2 results in gross cellular disorganization and HF-SC polarization within the niche. LHX2 loss leads to a failure to maintain HF-SC quiescence and hair anchoring, as well as progressive transformation of the niche into a sebaceous gland. These findings suggest that niche organization underlies the requirement for LHX2 in hair follicle structure and function.
AB - In adult skin, self-renewing, undifferentiated hair follicle stem cells (HF-SCs) reside within a specialized niche, where they spend prolonged times as a single layer of polarized, quiescent epithelial cells. When sufficient activating signals accumulate, HF-SCs become mobilized to fuel tissue regeneration and hair growth. Here, we show that architectural organization of the HF-SC niche by transcription factor LHX2 plays a critical role in HF-SC behavior. Using genome-wide chromatin and transcriptional profiling of HF-SCs in vivo, we show that LHX2 directly transactivates genes that orchestrate cytoskeletal dynamics and adhesion. Conditional ablation of LHX2 results in gross cellular disorganization and HF-SC polarization within the niche. LHX2 loss leads to a failure to maintain HF-SC quiescence and hair anchoring, as well as progressive transformation of the niche into a sebaceous gland. These findings suggest that niche organization underlies the requirement for LHX2 in hair follicle structure and function.
UR - http://www.scopus.com/inward/record.url?scp=84884143214&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884143214&partnerID=8YFLogxK
U2 - 10.1016/j.stem.2013.06.018
DO - 10.1016/j.stem.2013.06.018
M3 - Article
C2 - 24012369
AN - SCOPUS:84884143214
VL - 13
SP - 314
EP - 327
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 3
ER -