Approaches to the complete determination of the rotation axes and flexibility in the methylene segments of phospholipids: Deuterium, carbon-13, and proton nmr studies of dipalmitolyl phosphatidyl choline

Alan K. Engelt, David Cowburn

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Deuterium NMR has been widely used to investigate flexibility in phospholipids.’ Usually, the measured deuterium quadrupolar couplings are directly related to segmental motion via a single Saupe order parameter. A more complete description avoiding assumptions concerning orientation and modes of motion can be obtained from five order parameters for a particular segment. Employing this approach, we present experimental NMR measurements using isotopic isomers of dipalmitoyl phosphatidyl choline in bilayer dispersions for the two stereospecific deuterium quadrupole couplings,2 the carbon-proton dipole couplings, and, preliminarily, the proton-proton dipole couplings, for the C-2 methylene segment of the sn-2 chain of dipalmitoyl phosphatidyl choline. In addition, we discuss the relation of these couplings to the significant order parameters, and calculate tentative orientations of the sn -2 a methylene segment.

Original languageEnglish (US)
Pages (from-to)319-335
Number of pages17
JournalJournal of Biomolecular Structure and Dynamics
Issue number1
Publication statusPublished - Oct 1983
Externally publishedYes


ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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