Application of proteomic techniques to human tissues

Annely M. Richardson, Jaime Rodriguez-Canales, Gallya Gannot, Steven K. Libutti, Rodrigo F. Chuaqui, Michael A. Tangrea

Research output: Contribution to journalArticle

Abstract

Pathology has evolved over the past 30 years to incorporate molecular analysis of tissue samples for the assessment of disease states. With the advent of molecular pathology has come the development of new technologies to evaluate the tissue specimen at the molecular level. In this review, we will focus on proteomic analysis of human tissues. The importance of tissue preservation as well as the specific isolation of cell populations via microdissection will be addressed. In addition, the molecular evaluation of the tissue specimen with current proteomic techniques, including 2D polyacrylamide gel electrophoresis, Western blotting, protein arrays and mass spectrometry, will also be discussed. Finally, two new technologies will be introduced: direct tissue mass spectrometry and layered expression scanning. These new methods maintain the 2D architecture of the tissue throughout the proteomic analysis process, providing additional information that is typically lost with microdissected samples. The challenging application of these new molecular technologies to the analysis of human tissue specimens is essential for the better understanding of diseases.

Original languageEnglish (US)
Pages (from-to)110-118
Number of pages9
JournalJournal of Organ Dysfunction
Volume5
Issue number2
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Proteomics
Technology
Mass Spectrometry
Tissue Preservation
Protein Array Analysis
Microdissection
Molecular Pathology
Cell Separation
Electrophoresis, Gel, Two-Dimensional
Western Blotting
Pathology
Population

Keywords

  • 2D polyacrylamide gel electrophoresis
  • Mass spectrometry
  • Microdissection
  • Molecular pathology
  • Protein array
  • Proteomics
  • Tissue analysis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Critical Care
  • Physiology
  • Molecular Biology

Cite this

Richardson, A. M., Rodriguez-Canales, J., Gannot, G., Libutti, S. K., Chuaqui, R. F., & Tangrea, M. A. (2009). Application of proteomic techniques to human tissues. Journal of Organ Dysfunction, 5(2), 110-118. https://doi.org/10.1080/17471060701682203

Application of proteomic techniques to human tissues. / Richardson, Annely M.; Rodriguez-Canales, Jaime; Gannot, Gallya; Libutti, Steven K.; Chuaqui, Rodrigo F.; Tangrea, Michael A.

In: Journal of Organ Dysfunction, Vol. 5, No. 2, 2009, p. 110-118.

Research output: Contribution to journalArticle

Richardson, AM, Rodriguez-Canales, J, Gannot, G, Libutti, SK, Chuaqui, RF & Tangrea, MA 2009, 'Application of proteomic techniques to human tissues', Journal of Organ Dysfunction, vol. 5, no. 2, pp. 110-118. https://doi.org/10.1080/17471060701682203
Richardson AM, Rodriguez-Canales J, Gannot G, Libutti SK, Chuaqui RF, Tangrea MA. Application of proteomic techniques to human tissues. Journal of Organ Dysfunction. 2009;5(2):110-118. https://doi.org/10.1080/17471060701682203
Richardson, Annely M. ; Rodriguez-Canales, Jaime ; Gannot, Gallya ; Libutti, Steven K. ; Chuaqui, Rodrigo F. ; Tangrea, Michael A. / Application of proteomic techniques to human tissues. In: Journal of Organ Dysfunction. 2009 ; Vol. 5, No. 2. pp. 110-118.
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