TY - JOUR
T1 - Apoptotic process in the monkey small intestinal epithelium
T2 - I. Association with glutathione level and its efflux
AU - Benard, O.
AU - Madesh, M.
AU - Anup, R.
AU - Balasubramanian, K. A.
N1 - Funding Information:
Acknowledgements — The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology, Government of India is gratefully acknowledged. O. Benard (RA), M. Madesh (SRF) and Anup Ramachandran (JRF) are supported by the Council of Scientific and Industrial Research, India.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1999/2
Y1 - 1999/2
N2 - The apoptotic process in the normal gastrointestinal mucosa is of interest due to its possible role in physiological cell renewal. The aim of this study was to identify the apoptotic process in the monkey small intestine and the association of glutathione level and its efflux in this process. Monkey small intestinal epithelial cells were separated in to different fractions consisting of villus, middle and crypt cells. Apoptosis was identified by DNA ladder pattern and Hoechst staining. The level of glutathione, its efflux and the enzymes involved in its metabolism were quantitated in these fractions. Apoptotic cells were identified predominantly in the villus tip cell fractions by both DNA ladder pattern and Hoechst dye staining. Glutathione level was 7 fold higher in the crypt cells as compared to villus tip cells the middle cells showing a gradual decrease. A similar pattern was seen in mitochondrial content of glutathione. As the cells mature from crypt to villus, there is increased efflux of GSH, which may be responsible for the decreased level of GSH in apoptotic villus cells. In the monkey small intestine, apoptotic cells are seen in the villus tip fractions and the glutathione level and its efflux may play a role in this process.
AB - The apoptotic process in the normal gastrointestinal mucosa is of interest due to its possible role in physiological cell renewal. The aim of this study was to identify the apoptotic process in the monkey small intestine and the association of glutathione level and its efflux in this process. Monkey small intestinal epithelial cells were separated in to different fractions consisting of villus, middle and crypt cells. Apoptosis was identified by DNA ladder pattern and Hoechst staining. The level of glutathione, its efflux and the enzymes involved in its metabolism were quantitated in these fractions. Apoptotic cells were identified predominantly in the villus tip cell fractions by both DNA ladder pattern and Hoechst dye staining. Glutathione level was 7 fold higher in the crypt cells as compared to villus tip cells the middle cells showing a gradual decrease. A similar pattern was seen in mitochondrial content of glutathione. As the cells mature from crypt to villus, there is increased efflux of GSH, which may be responsible for the decreased level of GSH in apoptotic villus cells. In the monkey small intestine, apoptotic cells are seen in the villus tip fractions and the glutathione level and its efflux may play a role in this process.
KW - Apoptosis
KW - Glutathione efflux
KW - Intestinal epithelium
KW - Monkey
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U2 - 10.1016/S0891-5849(98)00164-6
DO - 10.1016/S0891-5849(98)00164-6
M3 - Article
C2 - 9895213
AN - SCOPUS:0032902405
SN - 0891-5849
VL - 26
SP - 245
EP - 252
JO - Free Radical Biology and Medicine
JF - Free Radical Biology and Medicine
IS - 3-4
ER -