Apical surface formation in MDCK cells: Regulation by the serine/threonine kinase EMK1

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

It has recently become evident that basic mechanisms for the establishment of cell polarity are conserved between epithelial and nonepithelial systems. The vast catalogue of known gene products involved in various aspects of invertebrate and yeast cell polarity provides a repertoire of candidate proteins that can be tested for their roles in the organization of mammalian epithelia. Here, we describe cell biological approaches to study the development and maintenance of cell polarity in Mardin-Darby canine kidney (MDCK) cells, an established mammalian model cell line for simple epithelia. The assays allowed us to characterize the Caenorhabditis elegans PAR-1 homologue EMK1 as a novel regulator of apical surface formation in epithelial cells.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalMethods
Volume30
Issue number3
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

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Cell Polarity
Protein-Serine-Threonine Kinases
Canidae
Cells
Kidney
Epithelium
Yeast
Assays
Genes
Caenorhabditis elegans
Invertebrates
Yeasts
Epithelial Cells
Maintenance
Cell Line
Proteins

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Apical surface formation in MDCK cells : Regulation by the serine/threonine kinase EMK1. / Cohen, David; Muesch, Anne.

In: Methods, Vol. 30, No. 3, 01.07.2003, p. 269-276.

Research output: Contribution to journalArticle

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