Apelin-induced vascular smooth muscle cell proliferation: The regulation of cyclin D1

Feng Li, Lanfang Li, Xuping Qin, Weinan Pan, Fen Feng, Feng Chen, Bingyan Zhu, Duanfang Liao, Herbert Tanowitz, Chris Albanese, Linxi Chen

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Apelin is the endogenous ligand of the G protein-coupled receptor, APJ. Vascular smooth muscle cells express both apelin and APJ, which are important regulatory factors in the cardiovascular and nervous systems. Importantly, APJ is also involved in the pathogenesis if HIV-1 infection. We investigated whether vascular smooth muscle cell proliferation was regulated through an apelin-pERK1/2-cyclin D1 signal transduction pathway. Apelin-13 significantly stimulated vascular smooth muscle cell proliferation and increased cell cycle progression. Apelin-13 a decreased the proportion of cell in the G0/G1 phase while increasing the number of cells in S phase. Apelin-13 also increased the levels of cyclin D1, cyclin E and pERK1/2. Treatment of cells with the MEK inhibitor PD98059 attenuated the apelin-3-induced pERK1/2 activation. Similarly, treatment with PD98059 partially diminished the apelin-13-induced expression of cyclin D1 and vascular smooth muscle cell proliferation. Taken together, these data established that apelin-13 stimulates vascular smooth muscle cell proliferation by promoting the G1-S phase transition, and that this effect is mediated in part by an apelin-pERKl/2-cyclin D1 signal cascade.

Original languageEnglish (US)
Pages (from-to)3786-3792
Number of pages7
JournalFrontiers in Bioscience
Volume13
Issue number10
DOIs
StatePublished - 2008

Fingerprint

Cyclin D1
Cell proliferation
Vascular Smooth Muscle
Smooth Muscle Myocytes
Muscle
Cell Proliferation
Cells
G1 Phase
S Phase
Cardiovascular system
Cyclin E
Signal transduction
Cell Cycle Resting Phase
Phase Transition
Mitogen-Activated Protein Kinase Kinases
Neurology
G-Protein-Coupled Receptors
Cardiovascular System
Nervous System
HIV Infections

Keywords

  • Apelin
  • APJ
  • Cell cycle
  • Cyclin d1
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Li, F., Li, L., Qin, X., Pan, W., Feng, F., Chen, F., ... Chen, L. (2008). Apelin-induced vascular smooth muscle cell proliferation: The regulation of cyclin D1. Frontiers in Bioscience, 13(10), 3786-3792. https://doi.org/10.2741/2967

Apelin-induced vascular smooth muscle cell proliferation : The regulation of cyclin D1. / Li, Feng; Li, Lanfang; Qin, Xuping; Pan, Weinan; Feng, Fen; Chen, Feng; Zhu, Bingyan; Liao, Duanfang; Tanowitz, Herbert; Albanese, Chris; Chen, Linxi.

In: Frontiers in Bioscience, Vol. 13, No. 10, 2008, p. 3786-3792.

Research output: Contribution to journalArticle

Li, F, Li, L, Qin, X, Pan, W, Feng, F, Chen, F, Zhu, B, Liao, D, Tanowitz, H, Albanese, C & Chen, L 2008, 'Apelin-induced vascular smooth muscle cell proliferation: The regulation of cyclin D1', Frontiers in Bioscience, vol. 13, no. 10, pp. 3786-3792. https://doi.org/10.2741/2967
Li, Feng ; Li, Lanfang ; Qin, Xuping ; Pan, Weinan ; Feng, Fen ; Chen, Feng ; Zhu, Bingyan ; Liao, Duanfang ; Tanowitz, Herbert ; Albanese, Chris ; Chen, Linxi. / Apelin-induced vascular smooth muscle cell proliferation : The regulation of cyclin D1. In: Frontiers in Bioscience. 2008 ; Vol. 13, No. 10. pp. 3786-3792.
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