APC/CCCS52A complexes control meristem maintenance in the Arabidopsis root

Marleen Vanstraelen, Mikhail Baloban, Olivier Da Ines, Antonietta Cultrone, Tim Lammens, Vé Ronique Boudolf, Spencer C. Brown, Lieven De Veylder, Peter Mergaert, Eva Kondorosi

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Plant organs originate from meristems where stem cells are maintained to produce continuously daughter cells that are the source of different cell types. The cell cycle switch gene CCS52A, a substrate specific activator of the anaphase promoting complex/ cyclosome (APC/C), controls the mitotic arrest and the transition of mitotic cycles to endoreduplication (ER) cycles as part of cell differentiation. Arabidopsis, unlike other organisms, contains 2 CCS52A isoforms. Here, we show that both of them are active and regulate meristem maintenance in the root tip, although through different mechanisms. The CCS52A1 activity in the elongation zone of the root stimulates ER and mitotic exit, and contributes to the border delineation between dividing and expanding cells. In contrast, CCS52A2 acts directly in the distal region of the root meristem to control identity of the quiescent center (QC) cells and stem cell maintenance. Cell proliferation assays in roots suggest that this control involves CCS52A2 mediated repression of mitotic activity in the QC cells. The data indicate that the CCS52A genes favor a low mitotic state in different cell types of the root tip that is required for meristem maintenance, and reveal a previously undescribed mechanism for APC/C mediated control in plant development.

Original languageEnglish (US)
Pages (from-to)11806-11811
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number28
DOIs
StatePublished - Jul 14 2009
Externally publishedYes

Fingerprint

Meristem
Arabidopsis
Maintenance
Endoreduplication
Anaphase-Promoting Complex-Cyclosome
Stem Cells
Switch Genes
cdc Genes
Plant Development
Cell Differentiation
Protein Isoforms
Cell Proliferation
Genes

Keywords

  • CDH1
  • Cell differentiation
  • Endoreduplication
  • Quiescent center
  • Stem cells

ASJC Scopus subject areas

  • General

Cite this

APC/CCCS52A complexes control meristem maintenance in the Arabidopsis root. / Vanstraelen, Marleen; Baloban, Mikhail; Da Ines, Olivier; Cultrone, Antonietta; Lammens, Tim; Boudolf, Vé Ronique; Brown, Spencer C.; De Veylder, Lieven; Mergaert, Peter; Kondorosi, Eva.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, No. 28, 14.07.2009, p. 11806-11811.

Research output: Contribution to journalArticle

Vanstraelen, M, Baloban, M, Da Ines, O, Cultrone, A, Lammens, T, Boudolf, VR, Brown, SC, De Veylder, L, Mergaert, P & Kondorosi, E 2009, 'APC/CCCS52A complexes control meristem maintenance in the Arabidopsis root', Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 28, pp. 11806-11811. https://doi.org/10.1073/pnas.0901193106
Vanstraelen, Marleen ; Baloban, Mikhail ; Da Ines, Olivier ; Cultrone, Antonietta ; Lammens, Tim ; Boudolf, Vé Ronique ; Brown, Spencer C. ; De Veylder, Lieven ; Mergaert, Peter ; Kondorosi, Eva. / APC/CCCS52A complexes control meristem maintenance in the Arabidopsis root. In: Proceedings of the National Academy of Sciences of the United States of America. 2009 ; Vol. 106, No. 28. pp. 11806-11811.
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