Antimicrobial and anti-inflammatory activity of chitosan-alginate nanoparticles: A targeted therapy for cutaneous pathogens

Adam J. Friedman, Jenny Phan, David O. Schairer, Jackson Champer, Min Qin, Aslan Pirouz, Karin Blecher Paz, Ami Oren, Phil T. Liu, Robert L. Modlin, Jenny Kim

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Advances in nanotechnology have demonstrated potential application of nanoparticles (NPs) for effective and targeted drug delivery. Here we investigated the antimicrobial and immunological properties and the feasibility of using NPs to deliver antimicrobial agents to treat a cutaneous pathogen. NPs synthesized with chitosan and alginate demonstrated a direct antimicrobial activity in vitro against Propionibacterium acnes, the bacterium linked to the pathogenesis of acne. By electron microscopy (EM) imaging, chitosan-alginate NPs were found to induce the disruption of the P. acnes cell membrane, providing a mechanism for the bactericidal effect. The chitosan-alginate NPs also exhibited anti-inflammatory properties as they inhibited P. acnes-induced inflammatory cytokine production in human monocytes and keratinocytes. Furthermore, benzoyl peroxide (BP), a commonly used antiacne drug, was effectively encapsulated in the chitosan-alginate NPs and demonstrated superior antimicrobial activity against P. acnes compared with BP alone while demonstrating less toxicity to eukaryotic cells. Together, these data suggest the potential utility of topical delivery of chitosan-alginate NP-encapsulated drug therapy for the treatment of dermatologic conditions with infectious and inflammatory components.

Original languageEnglish (US)
Pages (from-to)1231-1239
Number of pages9
JournalJournal of Investigative Dermatology
Volume133
Issue number5
DOIs
StatePublished - May 2013

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Chitosan
Pathogens
Nanoparticles
Anti-Inflammatory Agents
Propionibacterium acnes
Skin
Benzoyl Peroxide
Therapeutics
Drug therapy
Nanotechnology
Acne Vulgaris
Eukaryotic Cells
Cell membranes
Anti-Infective Agents
alginic acid
Keratinocytes
Pharmaceutical Preparations
Electron microscopy
Toxicity
Monocytes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Antimicrobial and anti-inflammatory activity of chitosan-alginate nanoparticles : A targeted therapy for cutaneous pathogens. / Friedman, Adam J.; Phan, Jenny; Schairer, David O.; Champer, Jackson; Qin, Min; Pirouz, Aslan; Blecher Paz, Karin; Oren, Ami; Liu, Phil T.; Modlin, Robert L.; Kim, Jenny.

In: Journal of Investigative Dermatology, Vol. 133, No. 5, 05.2013, p. 1231-1239.

Research output: Contribution to journalArticle

Friedman, AJ, Phan, J, Schairer, DO, Champer, J, Qin, M, Pirouz, A, Blecher Paz, K, Oren, A, Liu, PT, Modlin, RL & Kim, J 2013, 'Antimicrobial and anti-inflammatory activity of chitosan-alginate nanoparticles: A targeted therapy for cutaneous pathogens', Journal of Investigative Dermatology, vol. 133, no. 5, pp. 1231-1239. https://doi.org/10.1038/jid.2012.399
Friedman, Adam J. ; Phan, Jenny ; Schairer, David O. ; Champer, Jackson ; Qin, Min ; Pirouz, Aslan ; Blecher Paz, Karin ; Oren, Ami ; Liu, Phil T. ; Modlin, Robert L. ; Kim, Jenny. / Antimicrobial and anti-inflammatory activity of chitosan-alginate nanoparticles : A targeted therapy for cutaneous pathogens. In: Journal of Investigative Dermatology. 2013 ; Vol. 133, No. 5. pp. 1231-1239.
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