Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin

María Dolores Lozano-Baena, Inmaculada Tasset, Sara Obregón-Cano, Antonio De Haro-Bailon, Andrés Muñoz-Serrano, Ángeles Alonso-Moraga

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cruciferous vegetables are well known and worldwide consumed due to their health benefits and cancer prevention properties. As a desirable cruciferous plant, Ethiopian mustard (Brassica carinata A. Braun) and its glucosinolate sinigrin were tested in the in vivo Drosophila melanogaster (SMART) and the in vitro HL60 (human promyelocytic leukaemia cell line) systems. High performance liquid chromatography (HPLC) analysis of plant samples confirmed the presence of sinigrin as principal B. carinata glucosinolate. SMART was performed by feeding D. melanogaster larvae either with different concentrations of plant/compound samples or combining them with hydrogen peroxide (a potent oxidative mutagen) being both antimutagenics. HL60 assays showed the tumoricidal activity of plant samples (IC50 = 0.28 mg·mL-1) and the breakdown products of sinigrin hydrolysis (IC50 = 2.71 μM). Our results enhance the potential of B. carinata as health promoter and chemopreventive in both systems and the leading role of sinigrin in these effects.

Original languageEnglish (US)
Pages (from-to)15748-15765
Number of pages18
JournalMolecules
Volume20
Issue number9
DOIs
StatePublished - Sep 1 2015

Fingerprint

Brassica
Tumors
tumors
Glucosinolates
health
mutagens
Drosophila melanogaster
Inhibitory Concentration 50
larvae
Drosophila
Neoplasms
vegetables
leukemias
liquid chromatography
Health
hydrogen peroxide
cultured cells
Mustard Plant
hydrolysis
Vegetables

Keywords

  • Antigenotoxicity
  • Bioactive compound
  • Brassica
  • Cytotoxicity
  • Drosophila melanogaster
  • HL60
  • Sinigrin

ASJC Scopus subject areas

  • Medicine(all)
  • Organic Chemistry

Cite this

Lozano-Baena, M. D., Tasset, I., Obregón-Cano, S., De Haro-Bailon, A., Muñoz-Serrano, A., & Alonso-Moraga, Á. (2015). Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin. Molecules, 20(9), 15748-15765. https://doi.org/10.3390/molecules200915748

Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin. / Lozano-Baena, María Dolores; Tasset, Inmaculada; Obregón-Cano, Sara; De Haro-Bailon, Antonio; Muñoz-Serrano, Andrés; Alonso-Moraga, Ángeles.

In: Molecules, Vol. 20, No. 9, 01.09.2015, p. 15748-15765.

Research output: Contribution to journalArticle

Lozano-Baena, MD, Tasset, I, Obregón-Cano, S, De Haro-Bailon, A, Muñoz-Serrano, A & Alonso-Moraga, Á 2015, 'Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin', Molecules, vol. 20, no. 9, pp. 15748-15765. https://doi.org/10.3390/molecules200915748
Lozano-Baena MD, Tasset I, Obregón-Cano S, De Haro-Bailon A, Muñoz-Serrano A, Alonso-Moraga Á. Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin. Molecules. 2015 Sep 1;20(9):15748-15765. https://doi.org/10.3390/molecules200915748
Lozano-Baena, María Dolores ; Tasset, Inmaculada ; Obregón-Cano, Sara ; De Haro-Bailon, Antonio ; Muñoz-Serrano, Andrés ; Alonso-Moraga, Ángeles. / Antigenotoxicity and tumor growing inhibition by leafy Brassica carinata and sinigrin. In: Molecules. 2015 ; Vol. 20, No. 9. pp. 15748-15765.
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