Antigen-specific immune responses to influenza vaccine in utero

Deepa Rastogi, Chaodong Wang, Xia Mao, Cynthia Lendor, Paul B. Rothman, Rachel L. Miller

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Initial immune responses to allergens may occur before birth, thereby modulating the subsequent development of atopy. This paradigm remains controversial, however, due to the inability to identify antigen-specific T cells in cord blood. The advent of MHC tetramers has revolutionized the detection of antigen-specific T cells. Tetramer staining of cord blood after CMV infection has demonstrated that effective CD8+ antigen-specific immune responses can follow intrauterine viral infections. We hypothesized that sensitization to antigens occurs in utero in humans. We studied cord blood B and T cell immune responses following vaccination against influenza during pregnancy. Anti-Fluzone and anti-matrix protein IgM antibodies were detected in 38.5% (27 of 70) and 40.0% (28 of 70), respectively, of cord blood specimens. Using MHC tetramers, HA-specific CD4+ T cells were detected among 25.0% (3 of 12) and 42.9% (6 of 14) of cord blood specimens possessing DRB1*0101 and DRB1*0401 HLA types, respectively, and were detected even when the DRB1 HLA type was inherited from the father. Matrix protein-specific CD8+ T cells were detected among 10.0% (2 of 20) of HLA-A*0201+ newborns. These results suggest that B and T cell immune responses occur in the fetus following vaccination against influenza and have important implications for determining when immune responses to environmental exposures begin.

Original languageEnglish (US)
Pages (from-to)1637-1646
Number of pages10
JournalJournal of Clinical Investigation
Volume117
Issue number6
DOIs
StatePublished - Jun 1 2007

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Influenza Vaccines
Histocompatibility Antigens Class II
Fetal Blood
T-Lymphocytes
Antigens
Human Influenza
Vaccination
B-Lymphocytes
CD8 Antigens
HLA-DRB1 Chains
Environmental Exposure
Virus Diseases
Fathers
Allergens
Immunoglobulin M
Blood Cells
Proteins
Fetus
Parturition
Newborn Infant

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Rastogi, D., Wang, C., Mao, X., Lendor, C., Rothman, P. B., & Miller, R. L. (2007). Antigen-specific immune responses to influenza vaccine in utero. Journal of Clinical Investigation, 117(6), 1637-1646. https://doi.org/10.1172/JCI29466

Antigen-specific immune responses to influenza vaccine in utero. / Rastogi, Deepa; Wang, Chaodong; Mao, Xia; Lendor, Cynthia; Rothman, Paul B.; Miller, Rachel L.

In: Journal of Clinical Investigation, Vol. 117, No. 6, 01.06.2007, p. 1637-1646.

Research output: Contribution to journalArticle

Rastogi, D, Wang, C, Mao, X, Lendor, C, Rothman, PB & Miller, RL 2007, 'Antigen-specific immune responses to influenza vaccine in utero', Journal of Clinical Investigation, vol. 117, no. 6, pp. 1637-1646. https://doi.org/10.1172/JCI29466
Rastogi, Deepa ; Wang, Chaodong ; Mao, Xia ; Lendor, Cynthia ; Rothman, Paul B. ; Miller, Rachel L. / Antigen-specific immune responses to influenza vaccine in utero. In: Journal of Clinical Investigation. 2007 ; Vol. 117, No. 6. pp. 1637-1646.
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