Antigen presenting capacity of brain microvasculature in altered peptide ligand modulation of experimental allergic encephalomyelitis

Laura Santambrogio, M. Pakaski, M. L. Wong, B. Cipriani, C. F. Brosnan, M. B. Lees, M. E. Dorf

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Co-immunization with an altered peptide ligand (LR) partially protects SJL mice from proteolipid protein peptide 139-151-induced experimental allergic encephalomyelitis [Kuchroo, V.K., Greer, J.M., Kaul, D., Ishioka, G.Y., Franco, A., Sette, A., Sobel, R.A., Lees, M.B., 1994. A single TCR antagonist peptide inhibits experimental allergic encephalomyelitis mediated by a diverse T cell repertoire. J. Immunol. 153, 3326-3336; Santambrogio, L., Lees, M.B., Sobel, R.A., 1998. Altered peptide ligand modulation of experimental allergic encephalomyelitis: immune responses within the CNS. J. Neuroimmunol. 81, 1-13]. Clinical protection was noted despite extensive central nervous system inflammation observed after co-immunization with native and altered peptides. To extend our previous reports on this model, we now compare MHC class II expression and antigen presenting cell activity of cells associated with the blood-brain barrier in diseased and protected mice. Immunohistochemical studies identified MHC class II products on both the endothelial and microglial/macrophage populations. Ex vivo experiments suggested a correlation between the reduced clinical disease observed in the co-immunized mice and the antigen presenting activity of cells at the blood- brain barrier. The results suggest that antigen presenting activity is primarily mediated by macrophage-lineage cells of the central nervous system.

Original languageEnglish (US)
Pages (from-to)81-91
Number of pages11
JournalJournal of Neuroimmunology
Volume93
Issue number1-2
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Autoimmune Experimental Encephalomyelitis
Microvessels
Ligands
Antigens
Peptides
Brain
Antigen-Presenting Cells
Blood-Brain Barrier
Immunization
Central Nervous System
Macrophages
Histocompatibility Antigens Class II
Inflammation
T-Lymphocytes
Population

Keywords

  • Brain endothelial cells
  • Experimental allergic encephalomyelitis
  • Inflammation
  • Perivascular macrophages
  • Proteolipid protein

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Antigen presenting capacity of brain microvasculature in altered peptide ligand modulation of experimental allergic encephalomyelitis. / Santambrogio, Laura; Pakaski, M.; Wong, M. L.; Cipriani, B.; Brosnan, C. F.; Lees, M. B.; Dorf, M. E.

In: Journal of Neuroimmunology, Vol. 93, No. 1-2, 01.01.1999, p. 81-91.

Research output: Contribution to journalArticle

Santambrogio, Laura ; Pakaski, M. ; Wong, M. L. ; Cipriani, B. ; Brosnan, C. F. ; Lees, M. B. ; Dorf, M. E. / Antigen presenting capacity of brain microvasculature in altered peptide ligand modulation of experimental allergic encephalomyelitis. In: Journal of Neuroimmunology. 1999 ; Vol. 93, No. 1-2. pp. 81-91.
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