Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I: Analysis of anticardiolipin antibodies in leprosy

M. Hojnik, B. Gilburd, L. Ziporen, M. Blank, Y. Tomer, M. A. Scheinberg, A. Tincani, B. Rozman, Y. Shoenfeld

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

We studied the effect of β2-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine β2-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without β2-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of β2-GPI on the leprosy and APS sera binding to CL was confirmed using purified human β2-GPI. Enhanced binding was seen if β2-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against β2-GPI were found by ELISA. Leprosy anti-β2-GPI antibodies appear to be a separate antibody population recognizing only β2-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their β2-GPI requirement for binding to CL.

Original languageEnglish (US)
Pages (from-to)515-521
Number of pages7
JournalLupus
Volume3
Issue number6
DOIs
StatePublished - Jan 1 1994
Externally publishedYes

Keywords

  • Anticardiolipin antibodies
  • Antiphospholipid antibodies
  • Leprosy
  • β-glycoprotein 1

ASJC Scopus subject areas

  • Rheumatology

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