Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I: Analysis of anticardiolipin antibodies in leprosy

M. Hojnik, B. Gilburd, L. Ziporen, M. Blank, Yaron Tomer, M. A. Scheinberg, A. Tincani, B. Rozman, Y. Shoenfeld

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

We studied the effect of β2-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine β2-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without β2-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of β2-GPI on the leprosy and APS sera binding to CL was confirmed using purified human β2-GPI. Enhanced binding was seen if β2-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against β2-GPI were found by ELISA. Leprosy anti-β2-GPI antibodies appear to be a separate antibody population recognizing only β2-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their β2-GPI requirement for binding to CL.

Original languageEnglish (US)
Pages (from-to)515-521
Number of pages7
JournalLupus
Volume3
Issue number6
StatePublished - 1994
Externally publishedYes

Fingerprint

Anticardiolipin Antibodies
Leprosy
Glycoproteins
Enzyme-Linked Immunosorbent Assay
Infection
Serum
Antiphospholipid Syndrome
Antibodies
Immunoglobulin G

Keywords

  • β-glycoprotein 1
  • Anticardiolipin antibodies
  • Antiphospholipid antibodies
  • Leprosy

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Hojnik, M., Gilburd, B., Ziporen, L., Blank, M., Tomer, Y., Scheinberg, M. A., ... Shoenfeld, Y. (1994). Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I: Analysis of anticardiolipin antibodies in leprosy. Lupus, 3(6), 515-521.

Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I : Analysis of anticardiolipin antibodies in leprosy. / Hojnik, M.; Gilburd, B.; Ziporen, L.; Blank, M.; Tomer, Yaron; Scheinberg, M. A.; Tincani, A.; Rozman, B.; Shoenfeld, Y.

In: Lupus, Vol. 3, No. 6, 1994, p. 515-521.

Research output: Contribution to journalArticle

Hojnik, M, Gilburd, B, Ziporen, L, Blank, M, Tomer, Y, Scheinberg, MA, Tincani, A, Rozman, B & Shoenfeld, Y 1994, 'Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I: Analysis of anticardiolipin antibodies in leprosy', Lupus, vol. 3, no. 6, pp. 515-521.
Hojnik, M. ; Gilburd, B. ; Ziporen, L. ; Blank, M. ; Tomer, Yaron ; Scheinberg, M. A. ; Tincani, A. ; Rozman, B. ; Shoenfeld, Y. / Anticardiolipin antibodies in infections are heterogenous in their dependency on β2-glycoprotein I : Analysis of anticardiolipin antibodies in leprosy. In: Lupus. 1994 ; Vol. 3, No. 6. pp. 515-521.
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abstract = "We studied the effect of β2-GPI on binding of antibodies in sera from patients with leprosy and patients with the antiphospholipid syndrome (APS) to CL in enzyme-linked immunosorbent assays (ELISAs). Increased levels of IgG aCL were detected in 59 of 61 leprosy patients' sera by the standard aCL-ELISA in the presence of bovine β2-GPI and in 60 of the 61 leprosy patients' sera by the modified aCL-ELISA without β2-GPI. When tested by both aCL-ELISAs on the same plate, 10/31 leprosy sera and 9/10 APS sera bound better in the standard aCL-ELISA, 16/31 leprosy sera bound better in the modified aCL-ELISA and in five leprosy and one APS sera the difference was not significant. A dose-dependent enhancing effect of β2-GPI on the leprosy and APS sera binding to CL was confirmed using purified human β2-GPI. Enhanced binding was seen if β2-GPI was added either before or together with the test serum. In 11/61 leprosy sera increased levels of IgG antibodies against β2-GPI were found by ELISA. Leprosy anti-β2-GPI antibodies appear to be a separate antibody population recognizing only β2-GPI adsorbed on the ELISA plate. These results demonstrate heterogeneity of leprosy aCL with respect to their β2-GPI requirement for binding to CL.",
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