Antibody response to cryptococcus neoformans capsular polysaccharide glucuronoxylomannan in patients after solid-organ transplantation

Ziba Jalali, Lucky Ng, Nina Singh, Liise-anne Pirofski

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cryptococcosis is an important complication of solid-organ transplantation, but the risk factors for disease are poorly understood. The goal of this study was to investigate whether specific or nonspecific serum immunoglobulin levels determined in samples obtained before and after solid-organ transplantation differed in patients who did or did not develop cryptococcosis after transplantation. We analyzed pretransplantation sera from 25 subjects, 15 who subsequently developed cryptococcosis and 10 who did not, and posttransplantation sera from 24 subjects, 13 who developed cryptococcosis and 11 who did not. All subjects received a tacrolimus-based immunosuppressive regimen. Total immunoglobulin levels were measured by immunodiffusion, and Cryptococcus neoformans capsular polysaccharide glucuronoxylomannan (GXM)-specific serum antibody levels were determined by enzyme-linked immunosorbent assays. The results showed that solid-organ transplantation had a significant effect on total immunoglobulin and GXM-reactive antibody levels. GXM-reactive antibody levels differed in subjects who did and did not develop cryptococcosis. In pretransplant serum samples, the levels of GXM-reactive immunoglobulin M (IgM) were significantly lower in subjects who developed cryptococcosis after transplantation than in those who did not. For posttransplant serum samples, the levels of GXM-reactive IgM and IgG were significantly higher among the subjects who developed cryptococcosis than among those who did not. These findings suggest that perturbations in the preexisting antibody or B-cell repertoire and/or related to treatment of rejection, transplantation, or immunosuppressive therapy could translate into an increased risk for transplant-associated cryptococcosis.

Original languageEnglish (US)
Pages (from-to)740-746
Number of pages7
JournalClinical and Vaccine Immunology
Volume13
Issue number7
DOIs
StatePublished - Jul 2006

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Transplantation (surgical)
Cryptococcosis
Cryptococcus neoformans
Organ Transplantation
Antibody Formation
Polysaccharides
Antibodies
Immunoglobulins
Immunosuppressive Agents
Serum
Immunoglobulin M
Immunosorbents
Transplants
Tacrolimus
Transplantation
Assays
Immunoglobulin G
Cells
glucuronoxylomannan
Immunodiffusion

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Antibody response to cryptococcus neoformans capsular polysaccharide glucuronoxylomannan in patients after solid-organ transplantation. / Jalali, Ziba; Ng, Lucky; Singh, Nina; Pirofski, Liise-anne.

In: Clinical and Vaccine Immunology, Vol. 13, No. 7, 07.2006, p. 740-746.

Research output: Contribution to journalArticle

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