Antibody response to a synthetic peptide derived from the human papillomavirus type 6/11 L2 protein in recurrent respiratory papillomatosis: Correlation between southern blot hybridization, polymerase chain reaction, and serology

Ruth Tachezy, Eva Hamsikova, Jaroslav Valvoda, Marc Van Ranst, Jan Betka, Robert D. Burk, Vladimir Vonka

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Recurrent respiratory papillomatosis (RRP) is the most common benign tumour of the larynx, affecting both children and adults. We present a series of 25 patients, including 10 cases of juvenile multiple, 8 cases of adult solitary, and 7 cases of adult multiple RRP. Biopsy tissue from each patient was screened by Southern blot hybridization and polymerase chain reaction for the presence of human papillomavirus (HPV) DNA. Sera from patients and age‐ and sex‐matched controls were tested for the presence of HPV‐specific antibodies using a synthetic pep‐tide derived from the minor capsid protein (L2) of HPV 6/11. By Southern blot hybridization and/or polymerase chain reaction, biopsies from all patients were positive for HPV 6/11 DNA. There was no difference in antibody response between cases and controls. Female cases and controls had significantly higher antibody titers than male subjects. A correlation was observed between the HPV‐specific antibody level and the number of surgery‐necessitating recurrences. © 1994 Wiiey‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalJournal of Medical Virology
Volume42
Issue number1
DOIs
StatePublished - Jan 1994

Keywords

  • HPV
  • PCR
  • human papillomavirus
  • larynx
  • recurrent respiratory papillomatosis
  • serology

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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