Antibody-mediated internalization of B lymphocyte surface membrane immunoglobulin

A. S. Rosenthal, J. M. Davie, David L. Rosenstreich, Kerstin U. Cehrs

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The ultrastructural correlates of antibody-mediated internalization of membrane immunoglobulin determinants has been studied in the guinea pig using a horseradish peroxidase conjugate of rabbit anti-guinea pig immunoglobulin. We have observed that polar flow of ligand-induced micro-aggregated membrane immunoglobulin proceeds by local deformation of the plasma membrane into open-ended endocytic vesicles which in turn accumulate at the Golgi-associated pole prior to completion of endocytosis. 'Capping' does not appear to result from simple diffusion of the aggregates within the plane of the membrane. In addition, the question of whether anti-immunoglobulin can induce the formation of uropods on B lymphocytes in the guinea pig was examined. Formation of uropod-like structures on B lymphocytes appears to be factitious and induced by accumulation at the Golgi-associated cell pole of non-internalizable aggregates of membrane immunoglobulin linked to cell debris. These observations are interpreted as supporting the concept that spontaneous uropod formation by lymphocytes in the absence of anti-immunoglobulin reagents is a characteristic of antigen-reactive thymus-derived lymphocytes in the guinea pig.

Original languageEnglish (US)
Pages (from-to)317-329
Number of pages13
JournalExperimental Cell Research
Volume81
Issue number2
DOIs
StatePublished - 1973
Externally publishedYes

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B-Cell Antigen Receptors
Immunoglobulins
B-Lymphocytes
Membranes
Antibodies
Guinea Pigs
Lymphocytes
Transport Vesicles
Horseradish Peroxidase
Endocytosis
Thymus Gland
Cell Membrane
Rabbits
Ligands
Antigens

ASJC Scopus subject areas

  • Cell Biology

Cite this

Antibody-mediated internalization of B lymphocyte surface membrane immunoglobulin. / Rosenthal, A. S.; Davie, J. M.; Rosenstreich, David L.; Cehrs, Kerstin U.

In: Experimental Cell Research, Vol. 81, No. 2, 1973, p. 317-329.

Research output: Contribution to journalArticle

Rosenthal, A. S. ; Davie, J. M. ; Rosenstreich, David L. ; Cehrs, Kerstin U. / Antibody-mediated internalization of B lymphocyte surface membrane immunoglobulin. In: Experimental Cell Research. 1973 ; Vol. 81, No. 2. pp. 317-329.
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