Antibody-guided alpha radiation effectively damages fungal biofilms

L. R. Martinez, R. A. Bryan, C. Apostolidis, A. Morgenstern, A. Casadevall, E. Dadachova

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The use of indwelling medical devices-pacemakers, prosthetic joints, catheters-is rapidly growing and is often complicated by infections with biofilm-forming microbes that are resistant to antimicrobial agents and host defense mechanisms. We investigated for the first time the use of microbe-specific monoclonal antibodies (MAbs) as delivery vehicles for targeting biofilms with cytocidal radiation. MAb 18B7 (immunoglobulin G1 [IgG1]), which binds to capsular polysaccharides of the human pathogenic fungus Cryptococcus neoformans, penetrated cryptococcal biofilms, as shown by confocal microscopy. When the alpha radiation-emitter 213-Bismuth (213Bi) was attached to MAb 18B7 and the radiolabeled MAb was added to C. neoformans biofilms, there was a 50% reduction in biofilm metabolic activity. In contrast, when the IgM MAb 13F1 labeled with 213Bi was used there was no penetration of the fungal biofilm and no damage. Unlabeled 18B7, 213Bi-labeled nonspecific MAbs, and gamma and beta types of radiation did not have an effect on biofilms. The lack of efficacy of gamma and beta radiation probably reflects the radioprotective properties of polysaccharide biofilm matrix. Our results indicate that C. neoformans biofilms are susceptible to treatment with antibody-targeted alpha radiation, suggesting a novel option for the prevention or treatment of microbial biofilms on indwelling medical devices.

Original languageEnglish (US)
Pages (from-to)2132-2136
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume50
Issue number6
DOIs
StatePublished - Jun 2006

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Alpha Particles
Biofilms
Antibodies
Bismuth
Cryptococcus neoformans
Beta Particles
Gamma Rays
Polysaccharides
Monoclonal Antibodies
Equipment and Supplies
Anti-Infective Agents
Confocal Microscopy
Immunoglobulin M
Immunoglobulins
Fungi
Catheters
Joints

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Martinez, L. R., Bryan, R. A., Apostolidis, C., Morgenstern, A., Casadevall, A., & Dadachova, E. (2006). Antibody-guided alpha radiation effectively damages fungal biofilms. Antimicrobial Agents and Chemotherapy, 50(6), 2132-2136. https://doi.org/10.1128/AAC.00120-06

Antibody-guided alpha radiation effectively damages fungal biofilms. / Martinez, L. R.; Bryan, R. A.; Apostolidis, C.; Morgenstern, A.; Casadevall, A.; Dadachova, E.

In: Antimicrobial Agents and Chemotherapy, Vol. 50, No. 6, 06.2006, p. 2132-2136.

Research output: Contribution to journalArticle

Martinez, LR, Bryan, RA, Apostolidis, C, Morgenstern, A, Casadevall, A & Dadachova, E 2006, 'Antibody-guided alpha radiation effectively damages fungal biofilms', Antimicrobial Agents and Chemotherapy, vol. 50, no. 6, pp. 2132-2136. https://doi.org/10.1128/AAC.00120-06
Martinez LR, Bryan RA, Apostolidis C, Morgenstern A, Casadevall A, Dadachova E. Antibody-guided alpha radiation effectively damages fungal biofilms. Antimicrobial Agents and Chemotherapy. 2006 Jun;50(6):2132-2136. https://doi.org/10.1128/AAC.00120-06
Martinez, L. R. ; Bryan, R. A. ; Apostolidis, C. ; Morgenstern, A. ; Casadevall, A. ; Dadachova, E. / Antibody-guided alpha radiation effectively damages fungal biofilms. In: Antimicrobial Agents and Chemotherapy. 2006 ; Vol. 50, No. 6. pp. 2132-2136.
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