TY - JOUR
T1 - Antibody efficacy in murine pulmonary Cryptococcus neoformans infection
T2 - A role for nitric oxide
AU - Rivera, Johanna
AU - Mukherjee, Jean
AU - Weiss, Louis M.
AU - Casadevall, Arturo
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2002/4/1
Y1 - 2002/4/1
N2 - We investigated the pathogenesis of pulmonary Cryptococcus neoformans infection and passive Ab efficacy in mice deficient in inducible NO synthase (NOS2-/-) and the parental strain. Parental mice lived significantly longer than NOS2-/- mice after intratracheal infection, despite having a higher lung fungal burden. Administration of Ab reduced lung CFU in both NOS2-/- and parental mice, but prolonged survival and increased the inflammatory response only in parental mice. Ab administration was associated with increased serum nitrite and reduced polysaccharide levels in parental mice. Eosinophils were present in greater numbers in the lung of infected NOS2-/- mice than parental mice, irrespective of Ab administration. C. neoformans infection in NOS2-/- mice resulted in significantly higher levels of IFN-γ, monocyte chemoattractant protein-1, and macrophage-inflammatory protein-1α than parental mice. Ab administration had different effects on infected NOS2-/- and parental mice with respect to IFN-γ, monocoyte chemoattractant protein-1, and macrophage-inflammatory protein-1α levels. Ab administration increased lung levels of IFN-γ in parental mice and reduced levels in NOS2-/- mice. The results indicate that NO is involved in the regulation of cytokine expression in response to cryptococcal pneumonia and is necessary for Ab efficacy against C. neoformans in mice. Our findings indicate a complex relationship between Ab efficacy against C. neoformans and cytokine expression, underscoring the interdependency of cellular and humoral defense mechanisms.
AB - We investigated the pathogenesis of pulmonary Cryptococcus neoformans infection and passive Ab efficacy in mice deficient in inducible NO synthase (NOS2-/-) and the parental strain. Parental mice lived significantly longer than NOS2-/- mice after intratracheal infection, despite having a higher lung fungal burden. Administration of Ab reduced lung CFU in both NOS2-/- and parental mice, but prolonged survival and increased the inflammatory response only in parental mice. Ab administration was associated with increased serum nitrite and reduced polysaccharide levels in parental mice. Eosinophils were present in greater numbers in the lung of infected NOS2-/- mice than parental mice, irrespective of Ab administration. C. neoformans infection in NOS2-/- mice resulted in significantly higher levels of IFN-γ, monocyte chemoattractant protein-1, and macrophage-inflammatory protein-1α than parental mice. Ab administration had different effects on infected NOS2-/- and parental mice with respect to IFN-γ, monocoyte chemoattractant protein-1, and macrophage-inflammatory protein-1α levels. Ab administration increased lung levels of IFN-γ in parental mice and reduced levels in NOS2-/- mice. The results indicate that NO is involved in the regulation of cytokine expression in response to cryptococcal pneumonia and is necessary for Ab efficacy against C. neoformans in mice. Our findings indicate a complex relationship between Ab efficacy against C. neoformans and cytokine expression, underscoring the interdependency of cellular and humoral defense mechanisms.
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U2 - 10.4049/jimmunol.168.7.3419
DO - 10.4049/jimmunol.168.7.3419
M3 - Article
C2 - 11907100
AN - SCOPUS:0036533656
SN - 0022-1767
VL - 168
SP - 3419
EP - 3427
JO - Journal of Immunology
JF - Journal of Immunology
IS - 7
ER -