Antibodies to lymphotoxin α (LTα) and LTβ recognize different glial cell types in the central nervous system

Barbara Cannella, Irene Dougas Sizing, Christopher D. Benjamin, Jeffrey L. Browning, Cedric S. Raine

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The cytokine lymphotoxin (LT) is known to exist in two forms, secreted LTα and a membrane-bound LTα/β complex. LTα shares the same receptor as tumor necrosis factor a and LTβ is recognized by its receptor, LTβR. Since LT has been associated with oligodendrocyte pathology, the present study has examined the expression of these molecules by immunocytochemistry in diseased and normal CNS tissue, with a panel of monoclonal antibodies (mAb) to LTα, LTβ and LTβR. Of three mAb to LTβ, two (B27 and c37) gave specific membrane staining on astrocytes, as well as lymphocytes. The third anti-LTβ mAb, B9, was selectively immunoreactive for oligodendrocytes, suggesting specific recognition sites. The reactivity was not specific for multiple sclerosis (MS) since oligodendrocytes in normal and non-MS CNS tissue also displayed positivity. MAb to LTβR reacted with astrocytes only, giving a punctate membrane staining pattern suggestive of receptor sites. MAb to LTβ gave strong reactivity on lymphocytes in active MS lesions and weak reactivity on microglia within lesion areas. These results show that mAb to LTα and LTβ recognize different cell types within the CNS. Furthermore, individual mAb against LTβ were capable of distinguishing between astrocytes and oligodendrocytes, perhaps indicative of different epitopes on LTβ. The presence of LTβR on astrocytes suggests possible interactions between infiltrating lymphocytes and astrocytes via the LT pathway.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalJournal of Neuroimmunology
Volume78
Issue number1-2
DOIs
StatePublished - Sep 1997

Fingerprint

Lymphotoxin-alpha
Neuroglia
Central Nervous System
Antibodies
Astrocytes
Oligodendroglia
Monoclonal Antibodies
Lymphocytes
Multiple Sclerosis
Membranes
Staining and Labeling
Tumor Necrosis Factor Receptors
Central Nervous System Diseases
Microglia
Sclerosis

Keywords

  • Astrocytes
  • Central nervous system
  • Immunocytochemistry
  • Lymphotoxin
  • Multiple sclerosis
  • Oligodendrocytes

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Antibodies to lymphotoxin α (LTα) and LTβ recognize different glial cell types in the central nervous system. / Cannella, Barbara; Dougas Sizing, Irene; Benjamin, Christopher D.; Browning, Jeffrey L.; Raine, Cedric S.

In: Journal of Neuroimmunology, Vol. 78, No. 1-2, 09.1997, p. 172-179.

Research output: Contribution to journalArticle

Cannella, Barbara ; Dougas Sizing, Irene ; Benjamin, Christopher D. ; Browning, Jeffrey L. ; Raine, Cedric S. / Antibodies to lymphotoxin α (LTα) and LTβ recognize different glial cell types in the central nervous system. In: Journal of Neuroimmunology. 1997 ; Vol. 78, No. 1-2. pp. 172-179.
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N2 - The cytokine lymphotoxin (LT) is known to exist in two forms, secreted LTα and a membrane-bound LTα/β complex. LTα shares the same receptor as tumor necrosis factor a and LTβ is recognized by its receptor, LTβR. Since LT has been associated with oligodendrocyte pathology, the present study has examined the expression of these molecules by immunocytochemistry in diseased and normal CNS tissue, with a panel of monoclonal antibodies (mAb) to LTα, LTβ and LTβR. Of three mAb to LTβ, two (B27 and c37) gave specific membrane staining on astrocytes, as well as lymphocytes. The third anti-LTβ mAb, B9, was selectively immunoreactive for oligodendrocytes, suggesting specific recognition sites. The reactivity was not specific for multiple sclerosis (MS) since oligodendrocytes in normal and non-MS CNS tissue also displayed positivity. MAb to LTβR reacted with astrocytes only, giving a punctate membrane staining pattern suggestive of receptor sites. MAb to LTβ gave strong reactivity on lymphocytes in active MS lesions and weak reactivity on microglia within lesion areas. These results show that mAb to LTα and LTβ recognize different cell types within the CNS. Furthermore, individual mAb against LTβ were capable of distinguishing between astrocytes and oligodendrocytes, perhaps indicative of different epitopes on LTβ. The presence of LTβR on astrocytes suggests possible interactions between infiltrating lymphocytes and astrocytes via the LT pathway.

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