Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins

Yuko Tashima; Pamela Stanley

doi:10.1074/jbc.M113.492512

Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins

Yuko Tashima, Pamela Stanley

Cell Biology

Research output: Contribution to journal › Article

24 Scopus citations

Abstract

Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

Original language	English (US)
Pages (from-to)	11132-11142
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	289
Issue number	16
DOIs	https://doi.org/10.1074/jbc.M113.492512
State	Published - Apr 18 2014

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ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Cite this

Tashima, Y., & Stanley, P. (2014). Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins. Journal of Biological Chemistry, 289(16), 11132-11142. https://doi.org/10.1074/jbc.M113.492512

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AB - Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

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10.1074/jbc.M113.492512