TY - JOUR
T1 - Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins
AU - Tashima, Yuko
AU - Stanley, Pamela
PY - 2014/4/18
Y1 - 2014/4/18
N2 - Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.
AB - Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.
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U2 - 10.1074/jbc.M113.492512
DO - 10.1074/jbc.M113.492512
M3 - Article
C2 - 24573683
AN - SCOPUS:84899006820
VL - 289
SP - 11132
EP - 11142
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 16
ER -