Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins

Yuko Tashima, Pamela Stanley

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

Original languageEnglish (US)
Pages (from-to)11132-11142
Number of pages11
JournalJournal of Biological Chemistry
Volume289
Issue number16
DOIs
StatePublished - Apr 18 2014

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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