Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins

Yuko Tashima; Pamela Stanley

doi:10.1074/jbc.M113.492512

Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins

Yuko Tashima, Pamela Stanley

Cell Biology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

Original language	English (US)
Pages (from-to)	11132-11142
Number of pages	11
Journal	Journal of Biological Chemistry
Volume	289
Issue number	16
DOIs	https://doi.org/10.1074/jbc.M113.492512
State	Published - Apr 18 2014

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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title = "Antibodies that detect o-linked β-D-N-Acetylglucosamine on the extracellular domain of cell surface glycoproteins",

abstract = "Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.",

author = "Yuko Tashima and Pamela Stanley",

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AU - Tashima, Yuko

AU - Stanley, Pamela

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Y1 - 2014/4/18

N2 - Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

AB - Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.

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