Abstract
Background: Seven antibodies that detect cytosolic and nuclear O-GlcNAc were examined for recognition of O-GlcNAc on cell surface glycoproteins. Results: Three antibodies recognized cell surface O-GlcNAc and also bound terminal β-GlcNAc on N-glycans. Conclusion: With careful controls, cell surface O-GlcNAc can be detected using antibodies CTD110.6, 18B10.C7, and 9D1.E4. Significance: Antibodies to monitor cell surface O-GlcNAcylated proteins were characterized.
Original language | English (US) |
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Pages (from-to) | 11132-11142 |
Number of pages | 11 |
Journal | Journal of Biological Chemistry |
Volume | 289 |
Issue number | 16 |
DOIs | |
State | Published - Apr 18 2014 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology