Antibodies reactive to Non-HLA antigens in transplant glomerulopathy

Rajani Dinavahi, Ajish George, Anne Tretin, Enver Akalin, Scott Ames, Jonathan S. Bromberg, Graciela DeBoccardo, Nicholas DiPaola, Susan M. Lerner, Anita Mehrotra, Barbara T. Murphy, Tibor Nadasdy, Estela Paz-Artal, Daniel R. Salomon, Bernd Schröppel, Vinita Sehgal, Ravi Sachidanandam, Peter S. Heeger

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Although T and B cell alloimmunity contribute to transplant injury, autoimmunity directed at kidney-expressed, non-HLA antigens may also participate. Because the specificity, prevalence, and importance of antibodies to non-HLA antigens in late allograft injury are poorly characterized, we used a protein microarray to compare antibody repertoires in pre- and post-transplant sera from several cohorts of patients with and without transplant glomerulopathy. Transplantation routinely induced changes in antibody repertoires, but we did not identify any de novo non-HLA antibodies common to patients with transplant glomerulopathy. The screening studies identified three reactivities present before transplantation that persisted after transplant and strongly associated with transplant glomerulopathy. ELISA confirmed that reactivity against peroxisomal-trans-2-enoyl-coA-reductase strongly associated with the development of transplant glomerulopathy in independent validation sets. In addition to providing insight into effects of transplantation on non-HLA antibody repertoires, these results suggest that pretransplant serum antibodies to peroxisomal-trans-2-enoyl-coA-reductase may predict prognosis in kidney transplantation.

Original languageEnglish (US)
Pages (from-to)1168-1178
Number of pages11
JournalJournal of the American Society of Nephrology
Volume22
Issue number6
DOIs
StatePublished - Jun 2011

ASJC Scopus subject areas

  • Nephrology

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