TY - JOUR
T1 - Antibodies elicited by a cryptococcus neoformans-tetanus toxoid conjugate vaccine have the same specificity as those elicited in infection
AU - Casadevall, Arturo
AU - Mukherjee, Jean
AU - Devi, Sarvamangala J.N.
AU - Schneerson, Rachel
AU - Robbins, John B.
AU - Scharff, Matthew D.
N1 - Funding Information:
Financial support: National Institutes of Health (CA-39838. CA-13330. AI-10702; training grant 2T32C809173-16 to J.M.); Pfizer Postdoctoral Fellowship (to A.C.); the Harry Eagle Chair. provided by the Women's Division of Albert Einstein College of Medicine (to M.D.S.).
PY - 1992/6
Y1 - 1992/6
N2 - The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgGl monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgGl, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.
AB - The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). Infection rarely resulted in a rise of serum antibody titer to CNPS. In contrast, mice immunized with GXM-TT produced serum IgM and IgG to CNPS. Six IgM and one IgGl monoclonal antibodies (MAbs) were generated from the spleen of one infected mouse. Nine IgM, 1 IgG3, 16 IgGl, and 7 IgA MAbs were generated from the spleen of one GXM-TT-immunized mouse. All MAbs generated from both mice bound to the GXM fraction of the capsular polysaccharide. For some MAbs, de-O-acetylation of serotype A GXM abolished or greatly reduced MAb binding compared with the native GXM. All MAbs reacted with CNPS from C. neoformans serotypes A-D. MAbs generated from the infected mouse competitively inhibited the binding of MAbs generated from the GXM-TT-immunized mouse. These results indicate that some antibodies elicited by infection with C. neoformans or by immunization with GXM-TT bind to the same antigenic determinant in the GXM.
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U2 - 10.1093/infdis/165.6.1086
DO - 10.1093/infdis/165.6.1086
M3 - Article
C2 - 1583327
AN - SCOPUS:0026568749
SN - 0022-1899
VL - 165
SP - 1086
EP - 1093
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 6
ER -