Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status

Jacqueline M. Achkar, Elisabeth Jenny-Avital, Xian Yu, Susanne Burger, Eric Leibert, Patrick W. Bilder, Steven C. Almo, Arturo Casadevall, Suman Laal

Research output: Contribution to journalArticle

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Abstract

The immunodominance of Mycobacterium tuberculosis proteins malate synthase (MS) and MPT51 has been demonstrated in case-control studies with patients from countries in which tuberculosis (TB) is endemic. The value of these antigens for the serodiagnosis of TB now is evaluated in a cross-sectional study of pulmonary TB suspects in the United States diagnosed to have TB, HIV-associated TB, or other respiratory diseases (ORD). Serum antibody reactivity to recombinant purified MS and MPT51 was determined by enzyme-linked immunosorbent assays (ELISAs) of samples from TB suspects and well-characterized control groups. TB suspects were diagnosed with TB (n = 87; 49% sputum microscopy negative, 20% HIV+) or ORD (n = 63; 58% HIV+). Antibody reactivity to MS and MPT51 was significantly higher in U.S. HIV+/TB samples than in HIV-/TB samples (P < 0.001), and it was significantly higher in both TB groups than in control groups with latent TB infection (P < 0.001). Antibody reactivity to both antigens was higher in U.S. HIV+/TB samples than in HIV+/ORD samples (P = 0.052 for MS, P = 0.001 for MPT51) but not significantly different between HIV -/TB and HIV-/ORD. Among U.S. HIV+ TB suspects, a positive anti-MPT51 antibody response was strongly and significantly associated with TB (odds ratio, 11.0; 95% confidence interval, 2.3 to 51.2; P = 0.002). These findings have implications for the adjunctive use of TB serodiagnosis with these antigens in HIV+ subjects.

Original languageEnglish (US)
Pages (from-to)384-392
Number of pages9
JournalClinical and Vaccine Immunology
Volume17
Issue number3
DOIs
StatePublished - Mar 2010

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Malate Synthase
Immunodominant Epitopes
Pulmonary diseases
Mycobacterium tuberculosis
Tuberculosis
HIV
Antibodies
Antigens
Immunosorbents
Assays
Microscopic examination
Serologic Tests
Enzymes
Proteins
HIV Antigens
Latent Tuberculosis
Control Groups

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

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Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status. / Achkar, Jacqueline M.; Jenny-Avital, Elisabeth; Yu, Xian; Burger, Susanne; Leibert, Eric; Bilder, Patrick W.; Almo, Steven C.; Casadevall, Arturo; Laal, Suman.

In: Clinical and Vaccine Immunology, Vol. 17, No. 3, 03.2010, p. 384-392.

Research output: Contribution to journalArticle

Achkar, Jacqueline M. ; Jenny-Avital, Elisabeth ; Yu, Xian ; Burger, Susanne ; Leibert, Eric ; Bilder, Patrick W. ; Almo, Steven C. ; Casadevall, Arturo ; Laal, Suman. / Antibodies against immunodominant antigens of Mycobacterium tuberculosis in subjects with suspected tuberculosis in the United States compared by HIV status. In: Clinical and Vaccine Immunology. 2010 ; Vol. 17, No. 3. pp. 384-392.
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