Antibiotic supplementation of intraocular irrigating solutions

An in vitro model of antibacterial action

D. C. Gritz, A. V. Cevallos, G. Smolin, Jr Whitcher J.P., D. B. Jones

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Purpose: The addition of antibiotics to infusion solutions for cataract surgery is becoming increasingly popular. The authors developed an in vitro model to evaluate antibacterial effects of this use of antibiotics. Methods: Clinical isolates and/or reference strains of the following organisms were examined: coagulase-negative Staphylococcus spp, Staphylococcus aureus, Streptococcus pneumoniae, Streptococcus viridans, Streptococcus spp, Enterococcus spp, Proprionibacterium acnes, Moraxella nonliquifaciens, and Pseudomonas aeruginosa. Standardized suspensions of each organism were incubated with a control solution (Balanced Salt Solution) or Balanced Salt Solution containing the following antibiotics: vancomycin (20 μg/ml) or gentamicin (8 μg/ml) or gentamicin and vancomycin combined (8 and 20 μg/ml, respectively). Suspensions were incubated for 30, 60, and 120 minutes at room temperature. Samples were centrifuged, and the organisms were washed with Balanced Salt Solution before quantitative culturing. Each organism also was incubated for 48 hours in Mueller-Hinton broth with the same antibiotic concentrations. Results: Most of the organisms were not affected by exposure to the antibiotics for up to 140 minutes. P. aeruginosa and M. nonliquifaciens were exceptions, decreasing in colony numbers even with 30 minutes of exposure. Several Staphylococcus spp yielded variable results. All organisms demonstrated nearly complete inhibition of growth when exposed for an extended time to the appropriate antibiotic in broth. Conclusions: Exposure to antibiotics for a short period of time, such as during intraocular surgery, generally has no effect on organisms commonly responsible for endophthalmitis. The use of antibiotics in this manner should be critically reassessed until further study.

Original languageEnglish (US)
Pages (from-to)1204-1209
Number of pages6
JournalOphthalmology
Volume103
Issue number8
StatePublished - 1996
Externally publishedYes

Fingerprint

Anti-Bacterial Agents
Viridans Streptococci
Salts
Vancomycin
Gentamicins
Staphylococcus
Pseudomonas aeruginosa
Suspensions
Moraxella
Staphylococcal Pneumonia
Endophthalmitis
In Vitro Techniques
Coagulase
Acne Vulgaris
Enterococcus
Streptococcus pneumoniae
Cataract
Temperature
Growth

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Gritz, D. C., Cevallos, A. V., Smolin, G., Whitcher J.P., J., & Jones, D. B. (1996). Antibiotic supplementation of intraocular irrigating solutions: An in vitro model of antibacterial action. Ophthalmology, 103(8), 1204-1209.

Antibiotic supplementation of intraocular irrigating solutions : An in vitro model of antibacterial action. / Gritz, D. C.; Cevallos, A. V.; Smolin, G.; Whitcher J.P., Jr; Jones, D. B.

In: Ophthalmology, Vol. 103, No. 8, 1996, p. 1204-1209.

Research output: Contribution to journalArticle

Gritz, DC, Cevallos, AV, Smolin, G, Whitcher J.P., J & Jones, DB 1996, 'Antibiotic supplementation of intraocular irrigating solutions: An in vitro model of antibacterial action', Ophthalmology, vol. 103, no. 8, pp. 1204-1209.
Gritz DC, Cevallos AV, Smolin G, Whitcher J.P. J, Jones DB. Antibiotic supplementation of intraocular irrigating solutions: An in vitro model of antibacterial action. Ophthalmology. 1996;103(8):1204-1209.
Gritz, D. C. ; Cevallos, A. V. ; Smolin, G. ; Whitcher J.P., Jr ; Jones, D. B. / Antibiotic supplementation of intraocular irrigating solutions : An in vitro model of antibacterial action. In: Ophthalmology. 1996 ; Vol. 103, No. 8. pp. 1204-1209.
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