Anthocyanin-rich açaí (Euterpe oleracea Mart.) extract attenuates manganese-induced oxidative stress in rat primary astrocyte cultures

Vivian Da Silva Santos, Emily Bisen-Hersh, Yingchun Yu, Ingridy Simone Ribeiro Cabral, Viviani Nardini, Megan Culbreth, João Batista Teixeira Da Rocha, Fernando Barbosa, Michael Aschner

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Manganese (Mn) is an essential element for human health. However, at high concentrations Mn may be neurotoxic. Mn accumulates in astrocytes, affecting their redox status. In view of the high antioxidant and anti-inflammatory properties of the exotic Brazilian fruit açaí (Euterpe oleracea Mart.), its methanolic extract was obtained by solid-phase extraction (SPE). This açaíextract showed considerable anthocyanins content and direct antioxidant capacity. The açaíextract scavenged 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) with an EC50 of 19.1 ppm, showing higher antioxidant activity compared to butylated hydroxytoluene (BHT), but lower than ascorbic acid and quercetin. This obtained açaí extract also attenuated Mn-induced oxidative stress in primary cultured astrocytes. Specifically, the açaí extract at an optimal and nutritionally relevant concentration of 0.1 μg/ml prevented Mn-induced oxidative stress by (1) restoring GSH/GSSG ratio and net glutamate uptake, (2) protecting astrocytic membranes from lipid peroxidation, and (3) decreasing Mn-induced expression of erythroid 2-related factor (Nrf2) protein. A larger quantity of açaí extract exacerbated the effects of Mn on these parameters except with respect to lipid peroxidation assessed by means of F2-isoprostanes. These studies indicate that at nutritionally relevant concentration, anthocyanins obtained from açaí protect astrocytes against Mn neurotoxicity, but at high concentrations, the "pro- oxidant"effects of its constituents likely prevail. Future studies may be profitably directed at potential protective effects of açaí anthocyanins in nutraceutical formulations.

Original languageEnglish (US)
Pages (from-to)390-404
Number of pages15
JournalJournal of Toxicology and Environmental Health - Part A: Current Issues
Volume77
Issue number7
DOIs
StatePublished - Apr 3 2014

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Anthocyanins
Oxidative stress
Manganese
Astrocytes
Rats
Oxidative Stress
Antioxidants
Lipids
Lipid Peroxidation
F2-Isoprostanes
Butylated Hydroxytoluene
Glutathione Disulfide
Euterpe
Ascorbic acid
Quercetin
Solid Phase Extraction
Membrane Lipids
Dietary Supplements
Fruits
Oxidants

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Anthocyanin-rich açaí (Euterpe oleracea Mart.) extract attenuates manganese-induced oxidative stress in rat primary astrocyte cultures. / Santos, Vivian Da Silva; Bisen-Hersh, Emily; Yu, Yingchun; Cabral, Ingridy Simone Ribeiro; Nardini, Viviani; Culbreth, Megan; Da Rocha, João Batista Teixeira; Barbosa, Fernando; Aschner, Michael.

In: Journal of Toxicology and Environmental Health - Part A: Current Issues, Vol. 77, No. 7, 03.04.2014, p. 390-404.

Research output: Contribution to journalArticle

Santos, Vivian Da Silva ; Bisen-Hersh, Emily ; Yu, Yingchun ; Cabral, Ingridy Simone Ribeiro ; Nardini, Viviani ; Culbreth, Megan ; Da Rocha, João Batista Teixeira ; Barbosa, Fernando ; Aschner, Michael. / Anthocyanin-rich açaí (Euterpe oleracea Mart.) extract attenuates manganese-induced oxidative stress in rat primary astrocyte cultures. In: Journal of Toxicology and Environmental Health - Part A: Current Issues. 2014 ; Vol. 77, No. 7. pp. 390-404.
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abstract = "Manganese (Mn) is an essential element for human health. However, at high concentrations Mn may be neurotoxic. Mn accumulates in astrocytes, affecting their redox status. In view of the high antioxidant and anti-inflammatory properties of the exotic Brazilian fruit a{\cc}a{\'i} (Euterpe oleracea Mart.), its methanolic extract was obtained by solid-phase extraction (SPE). This a{\cc}a{\'i}extract showed considerable anthocyanins content and direct antioxidant capacity. The a{\cc}a{\'i}extract scavenged 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) with an EC50 of 19.1 ppm, showing higher antioxidant activity compared to butylated hydroxytoluene (BHT), but lower than ascorbic acid and quercetin. This obtained a{\cc}a{\'i} extract also attenuated Mn-induced oxidative stress in primary cultured astrocytes. Specifically, the a{\cc}a{\'i} extract at an optimal and nutritionally relevant concentration of 0.1 μg/ml prevented Mn-induced oxidative stress by (1) restoring GSH/GSSG ratio and net glutamate uptake, (2) protecting astrocytic membranes from lipid peroxidation, and (3) decreasing Mn-induced expression of erythroid 2-related factor (Nrf2) protein. A larger quantity of a{\cc}a{\'i} extract exacerbated the effects of Mn on these parameters except with respect to lipid peroxidation assessed by means of F2-isoprostanes. These studies indicate that at nutritionally relevant concentration, anthocyanins obtained from a{\cc}a{\'i} protect astrocytes against Mn neurotoxicity, but at high concentrations, the {"}pro- oxidant{"}effects of its constituents likely prevail. Future studies may be profitably directed at potential protective effects of a{\cc}a{\'i} anthocyanins in nutraceutical formulations.",
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AU - Santos, Vivian Da Silva

AU - Bisen-Hersh, Emily

AU - Yu, Yingchun

AU - Cabral, Ingridy Simone Ribeiro

AU - Nardini, Viviani

AU - Culbreth, Megan

AU - Da Rocha, João Batista Teixeira

AU - Barbosa, Fernando

AU - Aschner, Michael

PY - 2014/4/3

Y1 - 2014/4/3

N2 - Manganese (Mn) is an essential element for human health. However, at high concentrations Mn may be neurotoxic. Mn accumulates in astrocytes, affecting their redox status. In view of the high antioxidant and anti-inflammatory properties of the exotic Brazilian fruit açaí (Euterpe oleracea Mart.), its methanolic extract was obtained by solid-phase extraction (SPE). This açaíextract showed considerable anthocyanins content and direct antioxidant capacity. The açaíextract scavenged 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) with an EC50 of 19.1 ppm, showing higher antioxidant activity compared to butylated hydroxytoluene (BHT), but lower than ascorbic acid and quercetin. This obtained açaí extract also attenuated Mn-induced oxidative stress in primary cultured astrocytes. Specifically, the açaí extract at an optimal and nutritionally relevant concentration of 0.1 μg/ml prevented Mn-induced oxidative stress by (1) restoring GSH/GSSG ratio and net glutamate uptake, (2) protecting astrocytic membranes from lipid peroxidation, and (3) decreasing Mn-induced expression of erythroid 2-related factor (Nrf2) protein. A larger quantity of açaí extract exacerbated the effects of Mn on these parameters except with respect to lipid peroxidation assessed by means of F2-isoprostanes. These studies indicate that at nutritionally relevant concentration, anthocyanins obtained from açaí protect astrocytes against Mn neurotoxicity, but at high concentrations, the "pro- oxidant"effects of its constituents likely prevail. Future studies may be profitably directed at potential protective effects of açaí anthocyanins in nutraceutical formulations.

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