Antenatal maternal hypoxic stress

Adaptations of the placental renin-angiotensin system in the mouse

R. Goyal, R. Lister, A. Leitzke, D. Goyal, C. P. Gheorghe, L. D. Longo

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The stress of Antenatal Maternal Hypoxia (AMH) can lead to a number of physiological and pathological changes in both mother and fetus, changes which can be linked to alterations in placental morphology and gene regulation. Recently, in the Brown Norway rat "model" of placental insufficiency, we reported alterations in placental renin-angiotensin system (RAS) genes. Moreover, AMH can lead to reduced oxygen availability to the fetus, similar to a state of placental insufficiency. Thus, in pregnant mice dams we tested the hypothesis that antenatal maternal hypoxic stress leads to alterations in the placental RAS. These alterations may, in part, account for the phenotypic changes in both pregnant mice dams as well as fetus and adult offspring. Methods: Pregnant FVB/NJ mice dams were either maintained as controls, or exposed to 10.5% O 2 for 48 h from 15.5 to 17.5 day post coitum. We then measured placental mRNA and protein expression of several RAS genes (n = 4 to 5; P < 0.05 was considered significant). Result: In murine placenta: (1) angiotensinogen (AGT) mRNA was undetectable; however, AGT protein was detectable and increased significantly with AMH. (2) In AMH, although renin mRNA was reduced protein expression increased, in association with decreased microRNA (miRNA) 199b, which can lead to increased renin translation. (3) Also in AMH placenta, angiotensin converting enzyme (ACE) -1 mRNA was unaltered; however, protein expression increased significantly, in association with decreased miRNA 27a, which can result in increased ACE-1 translation. (4) In AMH placenta, ACE-2 mRNA was reduced significantly, whereas protein expression was significantly greater, in association with reduced miRNA 429. (5) In AMH placenta, angiotensin II type (AT) -1a receptor mRNA expression was unaltered while AT-1b receptor mRNA was undetectable in both groups. Moreover, AT-1 receptor protein expression was unchanged in response to AMH. (6) AT-2 receptor mRNA and proteins were undetectable in both groups. Conclusion: The normal murine placenta possesses several components of RAS, and in response to AMH several of these elements undergo important changes. In addition, differential expression of RAS mRNA, miRNA and protein, indicate post-transcriptional regulatory mechanisms involved with hypoxic stress, and necessitate further investigation.

Original languageEnglish (US)
Pages (from-to)134-139
Number of pages6
JournalPlacenta
Volume32
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Renin-Angiotensin System
Mothers
Messenger RNA
Placenta
MicroRNAs
Angiotensin Type 1 Receptor
Placental Insufficiency
Proteins
Angiotensinogen
Fetus
Peptidyl-Dipeptidase A
Renin
Angiotensin Type 2 Receptor
Pregnancy Proteins
Genes
Hypoxia
Oxygen

Keywords

  • Acclimatization
  • Angiotensin II
  • Developmental programming
  • Pre-eclampsia
  • Vasoconstriction

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology

Cite this

Goyal, R., Lister, R., Leitzke, A., Goyal, D., Gheorghe, C. P., & Longo, L. D. (2011). Antenatal maternal hypoxic stress: Adaptations of the placental renin-angiotensin system in the mouse. Placenta, 32(2), 134-139. https://doi.org/10.1016/j.placenta.2010.11.004

Antenatal maternal hypoxic stress : Adaptations of the placental renin-angiotensin system in the mouse. / Goyal, R.; Lister, R.; Leitzke, A.; Goyal, D.; Gheorghe, C. P.; Longo, L. D.

In: Placenta, Vol. 32, No. 2, 02.2011, p. 134-139.

Research output: Contribution to journalArticle

Goyal, R, Lister, R, Leitzke, A, Goyal, D, Gheorghe, CP & Longo, LD 2011, 'Antenatal maternal hypoxic stress: Adaptations of the placental renin-angiotensin system in the mouse', Placenta, vol. 32, no. 2, pp. 134-139. https://doi.org/10.1016/j.placenta.2010.11.004
Goyal, R. ; Lister, R. ; Leitzke, A. ; Goyal, D. ; Gheorghe, C. P. ; Longo, L. D. / Antenatal maternal hypoxic stress : Adaptations of the placental renin-angiotensin system in the mouse. In: Placenta. 2011 ; Vol. 32, No. 2. pp. 134-139.
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