Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosopnifa early embryos

David J. Sharp, Kristina R. Yu, John C. Sisson, William Sullivan, Jonathan M. Scholey

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

The positioning of centrosomes, or microtubule-organizing centres, within cells plays a critical part in animal development. Here we show that, in Drosophila embryos undergoing mitosis, the positioning of centrosomes within bipolar spindles and between daughter nuclei is determined by a balance of opposing forces generated by a bipolar kinesin motor, KLP61F, that is directed to microtubule plus ends, and a carboxy-terminal kinesin motor, Ncd, that is directed towards microtubule minus ends. This activity maintains the spacing between separated centrosomes during prometaphase and metaphase, and repositions centrosomes and daughter nuclei during late anaphase and telophase. Surprisingly, we do not observe a function for KLP61F in the initial separation of centrosomes during prophase. Our data indicate that KLP61F and Ncd may function by crosslinking and sliding antiparallel spindle microtubules in relation to one another, allowing KLP61F to push centrosomes apart and Ned to pull them together.

Original languageEnglish (US)
Pages (from-to)51-54
Number of pages4
JournalNature Cell Biology
Volume1
Issue number1
StatePublished - May 1999
Externally publishedYes

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Centrosome
Microtubules
Embryonic Structures
Kinesin
Microtubule-Organizing Center
Prometaphase
Telophase
Prophase
Anaphase
Metaphase
Mitosis
Drosophila

ASJC Scopus subject areas

  • Cell Biology

Cite this

Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosopnifa early embryos. / Sharp, David J.; Yu, Kristina R.; Sisson, John C.; Sullivan, William; Scholey, Jonathan M.

In: Nature Cell Biology, Vol. 1, No. 1, 05.1999, p. 51-54.

Research output: Contribution to journalArticle

Sharp, DJ, Yu, KR, Sisson, JC, Sullivan, W & Scholey, JM 1999, 'Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosopnifa early embryos', Nature Cell Biology, vol. 1, no. 1, pp. 51-54.
Sharp, David J. ; Yu, Kristina R. ; Sisson, John C. ; Sullivan, William ; Scholey, Jonathan M. / Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosopnifa early embryos. In: Nature Cell Biology. 1999 ; Vol. 1, No. 1. pp. 51-54.
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