Abstract
Chronic exposure of fetal mouse spinal cord-ganglion explants to the opioid antagonist naloxone (10 μM, 7 days) produced a pronounced upregulation of μ opioid receptors. The antagonist action was stereospecific, as it was produced by (-)-, but not by (+)-naloxone, and was dose-dependent. Half-maximal naloxone-induced receptor upregulation occurred after two days; receptor density was maximal at 5 days. Exposure of the explant cultures to naloxone (10 μM) in the presence of the protein synthesis inhibitor, cycloheximide (1 μM; a concentration which blocks >90% protein synthesis) resulted in receptor density changes that were similar to those observed in cultures exposed to naloxone alone. This finding suggests that antagonist-induced opiate receptor upregulation does not require the synthesis of new receptor molecules.
Original language | English (US) |
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Pages (from-to) | 287-291 |
Number of pages | 5 |
Journal | Developmental Brain Research |
Volume | 25 |
Issue number | 2 |
DOIs | |
State | Published - Mar 1986 |
Keywords
- naloxone
- opiate receptor
- receptor upregulation
- sensory ganglion
- spinal cord
- tissue culture
ASJC Scopus subject areas
- Developmental Neuroscience
- Developmental Biology