Anisotropy in sickle hemoglobin fibers from variations in bending and twist

M. S. Turner, R. W. Briehl, J. C. Wang, F. A. Ferrone, R. Josephs

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have studied the variations of twist and bend in sickle hemoglobin fibers. We find that these variations are consistent with an origin in equilibrium thermal fluctuations, which allows us to estimate the bending and torsional rigidities and effective corresponding material moduli. We measure bending by electron microscopy of frozen hydrated fibers and find that the bending persistence length, a measure of the length of fiber required before it starts to be significantly bent due to thermal fluctuations, is 130 μm, somewhat shorter than that previously reported using light microscopy. The torsional persistence length, obtained by re-analysis of previously published experiments, is found to be only 2.5 μm. Strikingly this means that the corresponding torsional rigidity of the fibers is only 6×10-27 J m, much less than their bending rigidity of 5×10-25 J m. For (normal) isotropic materials, one would instead expect these to be similar. Thus, we present the first quantitative evidence of a very significant material anisotropy in sickle hemoglobin fibers, as might arise from the difference between axial and lateral contacts within the fiber. We suggest that the relative softness of the fiber with respect to twist deformation contributes to the metastability of HbS fibers: HbS double strands are twisted in the fiber but not in the equilibrium crystalline state. Our measurements inform a theoretical model of the thermodynamic stability of fibers that takes account of both bending and extension/compression of hemoglobin (double) strands within the fiber.

Original languageEnglish (US)
Pages (from-to)1422-1427
Number of pages6
JournalJournal of Molecular Biology
Volume357
Issue number5
DOIs
StatePublished - Apr 14 2006

Fingerprint

Sickle Hemoglobin
Anisotropy
Hot Temperature
Thermodynamics
Microscopy
Electron Microscopy
Hemoglobins
Theoretical Models
Light

Keywords

  • Fiber
  • Fluctuations
  • Hemoglobin
  • Macrofiber
  • Sickle

ASJC Scopus subject areas

  • Virology

Cite this

Turner, M. S., Briehl, R. W., Wang, J. C., Ferrone, F. A., & Josephs, R. (2006). Anisotropy in sickle hemoglobin fibers from variations in bending and twist. Journal of Molecular Biology, 357(5), 1422-1427. https://doi.org/10.1016/j.jmb.2006.01.071

Anisotropy in sickle hemoglobin fibers from variations in bending and twist. / Turner, M. S.; Briehl, R. W.; Wang, J. C.; Ferrone, F. A.; Josephs, R.

In: Journal of Molecular Biology, Vol. 357, No. 5, 14.04.2006, p. 1422-1427.

Research output: Contribution to journalArticle

Turner, MS, Briehl, RW, Wang, JC, Ferrone, FA & Josephs, R 2006, 'Anisotropy in sickle hemoglobin fibers from variations in bending and twist', Journal of Molecular Biology, vol. 357, no. 5, pp. 1422-1427. https://doi.org/10.1016/j.jmb.2006.01.071
Turner, M. S. ; Briehl, R. W. ; Wang, J. C. ; Ferrone, F. A. ; Josephs, R. / Anisotropy in sickle hemoglobin fibers from variations in bending and twist. In: Journal of Molecular Biology. 2006 ; Vol. 357, No. 5. pp. 1422-1427.
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