TY - JOUR
T1 - Analysis of transcripts from 17p13.3 in medulloblastoma suggests ROX/MNT as a potential tumour suppressor gene
AU - Cvekl, Ales
AU - Zavadil, Jiri
AU - Birshtein, Barbara K.
AU - Grotzer, Michael A.
AU - Cvekl, Ales
N1 - Funding Information:
We thank the staff of the AECOM Biotechnology Center for the qPCR analysis and Dr. Dean Saghafi, Steve Young, and Irene Gruber for their support. We are grateful to Dr. I. Slavc, University Children’s Hospital of Vienna, Austria, and Dr. T. Shalaby, University Children’s Hospital of Zurich, Switzerland, for the collection of tumour samples. This work was partially supported by grants from the National Institutes of Health EY14237 (A.C.) and AI13509 (B.K.B.). A part of this work by A. Cvekl, Jr. was done at the Pelham Memorial High School, Research Project class, from 2002 to 2004.
PY - 2004/11
Y1 - 2004/11
N2 - Haploinsufficiency of the human 17p13.3 region is associated with 35% to 50% of medulloblastomas, indicating the presence of one or more tumour suppressor genes which have not yet been identified. Of 119 genes residing in this region, seven genes- 14-3-3ε (YWHAE), HIC-1, ROX/MNT (a helix-loop-helix transcription factor and member of the MYC/MAX superfamily), KIAA0399, UBE2G1 (ubiquitin ligase), ALOX15, and MINK - encode proteins with potential links to cancer. We investigated these genes and found significant levels of expression of ROX/MNT in adult human cerebellum, and in embryonic and postnatal mouse cerebellum. Six of 14 medulloblastomas showed a reduction of ROX/MNT expression, accompanied by a reduction of both UBE2G1 and 14-3-3ε in three tumours and a reduction of UBE2G1 in one tumour. Moreover, the relative expression of MYC to ROX/MNT was increased in 4 of the 14 medulloblastomas. Collectively, these data suggest that ROX/MNT should be considered a potential tumour suppressor gene in medulloblastoma.
AB - Haploinsufficiency of the human 17p13.3 region is associated with 35% to 50% of medulloblastomas, indicating the presence of one or more tumour suppressor genes which have not yet been identified. Of 119 genes residing in this region, seven genes- 14-3-3ε (YWHAE), HIC-1, ROX/MNT (a helix-loop-helix transcription factor and member of the MYC/MAX superfamily), KIAA0399, UBE2G1 (ubiquitin ligase), ALOX15, and MINK - encode proteins with potential links to cancer. We investigated these genes and found significant levels of expression of ROX/MNT in adult human cerebellum, and in embryonic and postnatal mouse cerebellum. Six of 14 medulloblastomas showed a reduction of ROX/MNT expression, accompanied by a reduction of both UBE2G1 and 14-3-3ε in three tumours and a reduction of UBE2G1 in one tumour. Moreover, the relative expression of MYC to ROX/MNT was increased in 4 of the 14 medulloblastomas. Collectively, these data suggest that ROX/MNT should be considered a potential tumour suppressor gene in medulloblastoma.
KW - Medulloblastoma
KW - Quantitative RT-PCR
KW - ROX/MNT
KW - c-MYC
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U2 - 10.1016/j.ejca.2004.08.005
DO - 10.1016/j.ejca.2004.08.005
M3 - Article
C2 - 15519529
AN - SCOPUS:7044260299
SN - 0959-8049
VL - 40
SP - 2525
EP - 2532
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 16
ER -