Analysis of the yeast peptidome and comparison with the human peptidome

Sayani Dasgupta, Ciyu Yang, Leandro M. Castro, Alexandre K. Tashima, Emer S. Ferro, Robyn D. Moir, Ian M. Willis, Lloyd D. Fricker

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peptides function as signaling molecules in species as diverse as humans and yeast. Mass spectrometry-based peptidomics techniques provide a relatively unbiased method to assess the peptidome of biological samples. In the present study, we used a quantitative peptidomic technique to characterize the peptidome of the yeast Saccharomyces cerevisiae and compare it to the peptidomes of mammalian cell lines and tissues. Altogether, 297 yeast peptides derived from 75 proteins were identified. The yeast peptides are similar to those of the human peptidome in average size and amino acid composition. Inhibition of proteasome activity with either bortezomib or epoxomicin led to decreased levels of some yeast peptides, suggesting that these peptides are generated by the proteasome. Approximately 30% of the yeast peptides correspond to the N- or C-terminus of the protein; the human peptidome is also highly represented in N- or C-terminal protein fragments. Most yeast and humans peptides are derived from a subset of abundant proteins, many with functions involving cellular metabolism or protein synthesis and folding. Of the 75 yeast proteins that give rise to peptides, 24 have orthologs that give rise to human and/or mouse peptides and for some, the same region of the proteins are found in the human, mouse, and yeast peptidomes. Taken together, these results support the hypothesis that intracellular peptides may have specific and conserved biological functions.

Original languageEnglish (US)
Article numbere0163312
JournalPLoS One
Volume11
Issue number9
DOIs
StatePublished - Sep 1 2016

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Yeast
Yeasts
peptides
yeasts
Peptides
Proteins
proteins
proteasome endopeptidase complex
Proteasome Endopeptidase Complex
Protein C
Fungal Proteins
protein folding
Protein Folding
mice
Set theory
amino acid composition
Metabolism
Mass spectrometry
Saccharomyces cerevisiae
quantitative analysis

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Analysis of the yeast peptidome and comparison with the human peptidome. / Dasgupta, Sayani; Yang, Ciyu; Castro, Leandro M.; Tashima, Alexandre K.; Ferro, Emer S.; Moir, Robyn D.; Willis, Ian M.; Fricker, Lloyd D.

In: PLoS One, Vol. 11, No. 9, e0163312, 01.09.2016.

Research output: Contribution to journalArticle

Dasgupta S, Yang C, Castro LM, Tashima AK, Ferro ES, Moir RD et al. Analysis of the yeast peptidome and comparison with the human peptidome. PLoS One. 2016 Sep 1;11(9). e0163312. https://doi.org/10.1371/journal.pone.0163312
Dasgupta, Sayani ; Yang, Ciyu ; Castro, Leandro M. ; Tashima, Alexandre K. ; Ferro, Emer S. ; Moir, Robyn D. ; Willis, Ian M. ; Fricker, Lloyd D. / Analysis of the yeast peptidome and comparison with the human peptidome. In: PLoS One. 2016 ; Vol. 11, No. 9.
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