Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics

Jonathan H. Wardman, Xin Zhang, Sandra Gagnon, Leandro M. Castro, Xiaorong Zhu, Donald F. Steiner, Robert Day, Lloyd D. Fricker

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Neuropeptides are produced from larger precursors by limited proteolysis, first by endopeptidases and then by carboxypeptidases. Major endopeptidases required for these cleavages include prohormone convertase (PC) 1/3 and PC2. In this study, quantitative peptidomics analysis was used to characterize the specific role PC1/3 plays in this process. Peptides isolated from hypothalamus, amygdala, and striatum of PC1/3 null mice were compared with those from heterozygous and wild-type mice. Extracts were labeled with stable isotopic tags and fractionated by HPLC, after which relative peptide levels were determined using tandem mass spectrometry. In total, 92 peptides were found, of which 35 were known neuropeptides or related peptides derived from 15 distinct secretory pathway proteins: 7B2, chromogranin A and B, cocaine- and amphetamine-regulated transcript, procholecystokinin, proenkephalin, promelanin concentrating hormone, proneurotensin, propituitary adenylate cyclase-activating peptide, proSAAS, prosomatosatin, provasoactive intestinal peptide, provasopressin, secretogranin III, and VGF. Among the peptides derived from these proteins, ∼1/3 were decreased in the PC1/3 null mice relative to wild-type mice, ∼1/3 showed no change, and ∼1/3 increased in PC1/3 null. Cleavage sites were analyzed in peptides that showed no change or that decreased in PC1/3 mice, and these results were compared with peptides that showed no change or decreased in previous peptidomic studies with PC2 null mice. Analysis of these sites showed that while PC1/3 and PC2 have overlapping substrate preferences, there are particular cleavage site residues that distinguish peptides preferred by each PC.

Original languageEnglish (US)
Pages (from-to)215-225
Number of pages11
JournalJournal of Neurochemistry
Volume114
Issue number1
DOIs
StatePublished - Jul 2010

Fingerprint

Proprotein Convertase 1
Brain
Peptides
Endopeptidases
Neuropeptides
Proprotein Convertase 2
Chromogranin B
Proprotein Convertases
Proteolysis
Carboxypeptidases
Chromogranin A
Secretory Pathway
Amphetamine
Tandem Mass Spectrometry
Amygdala
Cocaine
Adenylyl Cyclases
Hypothalamus
Mass spectrometry
Proteins

Keywords

  • Neuropeptide
  • Peptidase
  • Peptide processing
  • Proprotein convertase
  • ProSAAS
  • Protease

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics. / Wardman, Jonathan H.; Zhang, Xin; Gagnon, Sandra; Castro, Leandro M.; Zhu, Xiaorong; Steiner, Donald F.; Day, Robert; Fricker, Lloyd D.

In: Journal of Neurochemistry, Vol. 114, No. 1, 07.2010, p. 215-225.

Research output: Contribution to journalArticle

Wardman, Jonathan H. ; Zhang, Xin ; Gagnon, Sandra ; Castro, Leandro M. ; Zhu, Xiaorong ; Steiner, Donald F. ; Day, Robert ; Fricker, Lloyd D. / Analysis of peptides in prohormone convertase 1/3 null mouse brain using quantitative peptidomics. In: Journal of Neurochemistry. 2010 ; Vol. 114, No. 1. pp. 215-225.
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