Analysis of multiple components involved in the interaction between Cryptococcus neoformans and Acanthamoeba castellanii

Juliana Rizzo, Priscila C. Albuquerque, Julie M. Wolf, Renata Nascimento, Marcos D. Pereira, Joshua D. Nosanchuk, Marcio L. Rodrigues

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cryptococcus neoformans is an environmental fungus that can cause lethal meningoencephalitis in immunocompromised individuals. The mechanisms by which environmental microbes become pathogenic to mammals are still obscure, but different studies suggest that fungal virulence evolved from selection imposed by environmental predators. The soil-living Acanthamoeba castellanii is a well-known predator of C. neoformans. In this work, we evaluated the participation of C. neoformans virulence-associated structures in the interaction of fungal cells with A. castellanii. Fungal extracellular vesicles (EVs) and the polysaccharide glucuronoxylomannan (GXM) were internalized by A. castellanii with no impact on the viability of amoebal cells. EVs, but not free GXM, modulated antifungal properties of A. castellanii by inducing enhanced yeast survival. Phagocytosis of C. neoformans by amoebal cells and the pathogenic potential in a Galleria mellonella model were not affected by EVs, but previous interactions with A. castellanii rendered fungal cells more efficient in killing this invertebrate host. This observation was apparently associated with marked amoeba-induced changes in surface architecture and increased resistance to both oxygen- and nitrogen-derived molecular species. Our results indicate that multiple components with the potential to impact pathogenesis are involved in C. neoformans environmental interactions.

Original languageEnglish (US)
Pages (from-to)602-614
Number of pages13
JournalFungal Biology
Volume121
Issue number6-7
DOIs
StatePublished - Jun 1 2017

Fingerprint

Acanthamoeba castellanii
Cryptococcus neoformans
vesicle
virulence
predator
Virulence
polysaccharide
yeast
viability
immunocompromised population
predators
mammal
antifungal properties
invertebrate
Meningoencephalitis
fungus
Amoeba
meningoencephalitis
Galleria mellonella
cells

Keywords

  • Amoebal predators
  • Cell surface
  • Fungal virulence

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Plant Science
  • Infectious Diseases

Cite this

Analysis of multiple components involved in the interaction between Cryptococcus neoformans and Acanthamoeba castellanii. / Rizzo, Juliana; Albuquerque, Priscila C.; Wolf, Julie M.; Nascimento, Renata; Pereira, Marcos D.; Nosanchuk, Joshua D.; Rodrigues, Marcio L.

In: Fungal Biology, Vol. 121, No. 6-7, 01.06.2017, p. 602-614.

Research output: Contribution to journalArticle

Rizzo, Juliana ; Albuquerque, Priscila C. ; Wolf, Julie M. ; Nascimento, Renata ; Pereira, Marcos D. ; Nosanchuk, Joshua D. ; Rodrigues, Marcio L. / Analysis of multiple components involved in the interaction between Cryptococcus neoformans and Acanthamoeba castellanii. In: Fungal Biology. 2017 ; Vol. 121, No. 6-7. pp. 602-614.
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