Analysis of meiotic prophase I in live mouse spermatocytes

Meisha A. Morelli, Uwe Werling, Winfried Edelmann, Mark S. Roberson, Paula E. Cohen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Events occurring during meiotic prophase I are critical for the successful production of haploid gametes. Many prophase I events are mediated by a meiosis-specific structure called the synaptonemal complex. To date, the limited knowledge we have about the dynamics of these prophase I events in mice comes from fixed, two-dimensional preparations of meiotic cells making it impossible to study the three-dimensional (3D) arrangement of meiotic chromosomes. The current study involves the development of an imaging system to view prophase I events in live mammalian spermatocytes by generating a transgenic mouse, Sycp3-Eyfp 21HC , expressing a fluorescently tagged synaptonemal complex protein, SYCP3. Using this live imaging system, the 3D structural arrangement of chromosomes in the different prophase I substages has been characterized in live spermatocytes, and aspects of the 3D architecture of spermatocytes have been observed that would not be possible with existing techniques. Additionally, chromosome movement in prophase I spermatocytes and meiotic progression from pachynema to diplonema were observed following treatment with the phosphatase inhibitor, okadaic acid (OA), which accelerates the progression of cells through late prophase I. These studies demonstrate that the Sycp3-Eyfp 21HC live imaging system is a useful tool for the study of mammalian prophase I dynamics.

Original languageEnglish (US)
Pages (from-to)743-760
Number of pages18
JournalChromosome Research
Volume16
Issue number5
DOIs
StatePublished - Aug 2008

Fingerprint

Meiotic Prophase I
Spermatocytes
Synaptonemal Complex
Chromosomes
Pachytene Stage
Okadaic Acid
Haploidy
Meiosis
Phosphoric Monoester Hydrolases
Germ Cells
Transgenic Mice

Keywords

  • Live imaging
  • Meiosis
  • Mouse
  • Spermatocyte
  • Synaptonemal complex

ASJC Scopus subject areas

  • Genetics

Cite this

Morelli, M. A., Werling, U., Edelmann, W., Roberson, M. S., & Cohen, P. E. (2008). Analysis of meiotic prophase I in live mouse spermatocytes. Chromosome Research, 16(5), 743-760. https://doi.org/10.1007/s10577-008-1224-8

Analysis of meiotic prophase I in live mouse spermatocytes. / Morelli, Meisha A.; Werling, Uwe; Edelmann, Winfried; Roberson, Mark S.; Cohen, Paula E.

In: Chromosome Research, Vol. 16, No. 5, 08.2008, p. 743-760.

Research output: Contribution to journalArticle

Morelli, MA, Werling, U, Edelmann, W, Roberson, MS & Cohen, PE 2008, 'Analysis of meiotic prophase I in live mouse spermatocytes', Chromosome Research, vol. 16, no. 5, pp. 743-760. https://doi.org/10.1007/s10577-008-1224-8
Morelli, Meisha A. ; Werling, Uwe ; Edelmann, Winfried ; Roberson, Mark S. ; Cohen, Paula E. / Analysis of meiotic prophase I in live mouse spermatocytes. In: Chromosome Research. 2008 ; Vol. 16, No. 5. pp. 743-760.
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