Analysis of limb morphogenesis in a model system

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Abstract

A method for analysis of chicken limb morphogenesis was devised. This method consisted of grafting a limb ectodermal jacket containing dissociated and pelleted mesenchymal cellular components to the host somites. Different cellular components stuffed into the ectoderm could be mixed in varied ratios. After 7 days the grafts were analyzed for outgrowth. Stage 19 mesoblast cells alone when treated as above gave limblike outgrowths with good digits, toes, and claws in all cases. However, mesoblasts from the proximal half of older limbs (stages 24, 25 and chondrocytes) gave no outgrowths, and those from stage 23 gave outgrowths in 9% of the cases. In mixtures of 5% stage 19 cells with 95% chondrocytes, consistent morphogenesis (i.e., in 65% of grafts) occurred. The amount of morphogenesis (size of graft and perfection of digits) was directly proportional to the amount of stage 19 cells. However, these cells mixed with proximal cells of stages 23, 24, or 25 required higher proportions for equivalent morphogenesis. To obtain morphogenesis equivalent to the 5% mixture with chondrocytes, 10% stage 19 cells were needed when mixed with proximal stage 23, 25% with proximal stage 24 and 7% with proximal stage 25. Mixtures of stage 19 cells added to nonlimb (flank, stage 19) mesoderm, formed large tumorous mounds of tissue with no limblike features.

Original languageEnglish (US)
Pages (from-to)113-122
Number of pages10
JournalDevelopmental Biology
Volume28
Issue number1
DOIs
StatePublished - May 1972
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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