TY - JOUR
T1 - Analysis of limb morphogenesis in a model system
AU - Singer, Robert H.
N1 - Funding Information:
‘This research was completed under the direction of Professor Zwilling in partial fulfillment of the requirements for the degree of Doctor of Philosophy. The years I spent with Edgar Zwilling bring to memory his kindness, integrity, and inspiration, and I dedicate my contributions in this work to his memory. Supported by Grants Tl-HD-22 and HD-03465 from the National Institute of Child Health and Human Development. ‘Present address: Department of Biology, 16. 839, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139.
PY - 1972/5
Y1 - 1972/5
N2 - A method for analysis of chicken limb morphogenesis was devised. This method consisted of grafting a limb ectodermal jacket containing dissociated and pelleted mesenchymal cellular components to the host somites. Different cellular components stuffed into the ectoderm could be mixed in varied ratios. After 7 days the grafts were analyzed for outgrowth. Stage 19 mesoblast cells alone when treated as above gave limblike outgrowths with good digits, toes, and claws in all cases. However, mesoblasts from the proximal half of older limbs (stages 24, 25 and chondrocytes) gave no outgrowths, and those from stage 23 gave outgrowths in 9% of the cases. In mixtures of 5% stage 19 cells with 95% chondrocytes, consistent morphogenesis (i.e., in 65% of grafts) occurred. The amount of morphogenesis (size of graft and perfection of digits) was directly proportional to the amount of stage 19 cells. However, these cells mixed with proximal cells of stages 23, 24, or 25 required higher proportions for equivalent morphogenesis. To obtain morphogenesis equivalent to the 5% mixture with chondrocytes, 10% stage 19 cells were needed when mixed with proximal stage 23, 25% with proximal stage 24 and 7% with proximal stage 25. Mixtures of stage 19 cells added to nonlimb (flank, stage 19) mesoderm, formed large tumorous mounds of tissue with no limblike features.
AB - A method for analysis of chicken limb morphogenesis was devised. This method consisted of grafting a limb ectodermal jacket containing dissociated and pelleted mesenchymal cellular components to the host somites. Different cellular components stuffed into the ectoderm could be mixed in varied ratios. After 7 days the grafts were analyzed for outgrowth. Stage 19 mesoblast cells alone when treated as above gave limblike outgrowths with good digits, toes, and claws in all cases. However, mesoblasts from the proximal half of older limbs (stages 24, 25 and chondrocytes) gave no outgrowths, and those from stage 23 gave outgrowths in 9% of the cases. In mixtures of 5% stage 19 cells with 95% chondrocytes, consistent morphogenesis (i.e., in 65% of grafts) occurred. The amount of morphogenesis (size of graft and perfection of digits) was directly proportional to the amount of stage 19 cells. However, these cells mixed with proximal cells of stages 23, 24, or 25 required higher proportions for equivalent morphogenesis. To obtain morphogenesis equivalent to the 5% mixture with chondrocytes, 10% stage 19 cells were needed when mixed with proximal stage 23, 25% with proximal stage 24 and 7% with proximal stage 25. Mixtures of stage 19 cells added to nonlimb (flank, stage 19) mesoderm, formed large tumorous mounds of tissue with no limblike features.
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U2 - 10.1016/0012-1606(72)90130-3
DO - 10.1016/0012-1606(72)90130-3
M3 - Article
C2 - 5041189
AN - SCOPUS:0015345068
SN - 0012-1606
VL - 28
SP - 113
EP - 122
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -