Analysis of interacting cell populations in cultures of marrow from patients with neutropenia

J. S. Senn; H. A. Messner; E. R. Stanley

doi:10.1182/blood.v44.1.33.33

Analysis of interacting cell populations in cultures of marrow from patients with neutropenia

J. S. Senn, H. A. Messner, E. R. Stanley

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Utilizing an adherence separation procedure, marrow cells from six neutropenic patients were studied in cell culture for colony forming ability (CFU C), and for colony stimulating function of the adherent cell (A C) population. Assays on 2 patients showed reduced CFU C and A C function, while assays on 3 patients yielded normal CFU C and A C function; studies on the remaining patient exhibited reduced CFU C in the presence of normal A C function. The demonstration of these various abnormalities in marrow cell culture from neutropenic patients suggests that the in vitro techniques have relevance to the study of granulopoiesis in vivo.

Original language	English (US)
Pages (from-to)	33-40
Number of pages	8
Journal	Blood
Volume	44
Issue number	1
DOIs	https://doi.org/10.1182/blood.v44.1.33.33
State	Published - 1974
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Immunology
Hematology
Cell Biology

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10.1182/blood.v44.1.33.33

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abstract = "Utilizing an adherence separation procedure, marrow cells from six neutropenic patients were studied in cell culture for colony forming ability (CFU C), and for colony stimulating function of the adherent cell (A C) population. Assays on 2 patients showed reduced CFU C and A C function, while assays on 3 patients yielded normal CFU C and A C function; studies on the remaining patient exhibited reduced CFU C in the presence of normal A C function. The demonstration of these various abnormalities in marrow cell culture from neutropenic patients suggests that the in vitro techniques have relevance to the study of granulopoiesis in vivo.",

author = "Senn, {J. S.} and Messner, {H. A.} and Stanley, {E. R.}",

year = "1974",

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AU - Messner, H. A.

AU - Stanley, E. R.

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N2 - Utilizing an adherence separation procedure, marrow cells from six neutropenic patients were studied in cell culture for colony forming ability (CFU C), and for colony stimulating function of the adherent cell (A C) population. Assays on 2 patients showed reduced CFU C and A C function, while assays on 3 patients yielded normal CFU C and A C function; studies on the remaining patient exhibited reduced CFU C in the presence of normal A C function. The demonstration of these various abnormalities in marrow cell culture from neutropenic patients suggests that the in vitro techniques have relevance to the study of granulopoiesis in vivo.

AB - Utilizing an adherence separation procedure, marrow cells from six neutropenic patients were studied in cell culture for colony forming ability (CFU C), and for colony stimulating function of the adherent cell (A C) population. Assays on 2 patients showed reduced CFU C and A C function, while assays on 3 patients yielded normal CFU C and A C function; studies on the remaining patient exhibited reduced CFU C in the presence of normal A C function. The demonstration of these various abnormalities in marrow cell culture from neutropenic patients suggests that the in vitro techniques have relevance to the study of granulopoiesis in vivo.

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Analysis of interacting cell populations in cultures of marrow from patients with neutropenia

Abstract

ASJC Scopus subject areas

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