Analysis of hepatocyte distribution and survival in vascular beds with cells marked by (99m)TC or endogenous dipeptidyl peptidase iv activity

Sanjeev Gupta, Srinivasa Rao G Vasa, Pankaj Rajvanshi, Lionel S. Zuckier, Christopher J. Palestro, Kuldeep K. Bhargava

Research output: Contribution to journalArticle

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Abstract

Knowledge of the kinetics of cell distribution in vascular beds will help optimize engraftment of transplanted hepatocytes. To noninvasively localize transplanted cells in vivo, we developed conditions for labeling rat hepatocytes with (99m)Tc-pertechnetate. The incorporated (99m)Tc was bound to intracellular proteins and did not impair cell viability. When (99m)Tc hcpatocytes were intrasplenically injected into normal rats, cells entered liver sinusoids with time-activity curves demonstrating instantaneous cell translocations. (99m)Tc activity in removed organs was in liver or spleen, and lungs showed little activity. However, when cells were intrasplenically transplanted into rats with portasystemic collaterals, (99m)Tc appeared in both liver sinusoids and pulmonary alveolar capillaries. To further localize cells, we transplanted DPPIV + F344 rat hepatocytes into syngeneic DPPIV - recipients. Histochemical staining for DPPIV activity demonstrated engraftment of intrasplenically transplanted cells in liver parenchyma. In contrast, when (99m)TC hepatocytes were injected into a peripheral vein, cells were entrapped in pulmonary capillaries but were subsequently broken down with redistribution of (99m)Tc activity elsewhere. Intact DPPIV + hepatocytes were identified in lungs, whereas only cell fragments were present in liver, spleen, or kidneys. These findings indicate that although the pulmonary vascular bed offers advantages of easy accessibility and a relatively large capacity, significant early cell destruction is an important limitation.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalCell Transplantation
Volume6
Issue number4
DOIs
StatePublished - Jul 1997

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Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
Liver
Blood Vessels
Hepatocytes
Rats
Lung
Cells
Labeling
Spleen
Peptide Hydrolases
Proteins
Sodium Pertechnetate Tc 99m
Kinetics
Inbred F344 Rats
Veins
Cell Survival

Keywords

  • (99m)Tc
  • Cell distribution
  • Endogenous dipeptidyl peptide IV
  • Vascular beds

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Analysis of hepatocyte distribution and survival in vascular beds with cells marked by (99m)TC or endogenous dipeptidyl peptidase iv activity. / Gupta, Sanjeev; Vasa, Srinivasa Rao G; Rajvanshi, Pankaj; Zuckier, Lionel S.; Palestro, Christopher J.; Bhargava, Kuldeep K.

In: Cell Transplantation, Vol. 6, No. 4, 07.1997, p. 377-386.

Research output: Contribution to journalArticle

Gupta, Sanjeev ; Vasa, Srinivasa Rao G ; Rajvanshi, Pankaj ; Zuckier, Lionel S. ; Palestro, Christopher J. ; Bhargava, Kuldeep K. / Analysis of hepatocyte distribution and survival in vascular beds with cells marked by (99m)TC or endogenous dipeptidyl peptidase iv activity. In: Cell Transplantation. 1997 ; Vol. 6, No. 4. pp. 377-386.
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