Analysis of cytoskeleton dynamics and cell migration in Drosophila ovaries using GFP-actin and E-cadherin-GFP fusion molecules

Vladyslav V. Verkhusha, Shoichiro Tsukita, Hiroki Oda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

Coordination of cell migration and adhesion is essential for movement of tissues during morphogenesis. During Drosophila oogenesis so called border cells (BCs) break from an anterior epithelium of egg chamber, acquire a mesenchymal-like morphology, and migrate posteriorly between nurse cells to oocyte. The confocal microscopic observation of BCs has revealed well-developed forepart lamellipodium stained with Drosophila E-cadherin (DE-cadherin), PS2 integrin, cytoplasmic myosin and F-actin. To investigate mechanism of BC migration in vivo we have constructed a DE-cadherin-GFP and a GFP-actin fusion proteins and induced their expression in BCs utilizing the UAS/GAL4 system. The DE-cadherin-GFP signal as well as immunostaining of PS2 integrin visualized a track of migrating BCs providing an evidence that adhesive molecules are pulled out and left behind on the surface of nurse cells. Our data suggest that two distinct adhesive systems, DE-cadherins and PS2 integrins simultaneously mediate the migration of BCs. Release of adhesive contacts in the tail region is a rate-limited event in BC migration. The spatial-temporal sequence of actin-based events visualized by the GFP-actin suggest a treadmilling model for actin behavior in BC lamellipodium. BC migration can be considered as simultaneous reiterating processes of lamellipodium extension and adhesive attachment, cytoskeletal contraction, and rear detachment.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages130-139
Number of pages10
ISBN (Print)0819430749
StatePublished - Jan 1 1999
Externally publishedYes
EventProceedings of the 1999 Optical Diagnostics of Living cells - San Jose, CA, USA
Duration: Jan 25 1999Jan 26 1999

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume3604
ISSN (Print)0277-786X

Other

OtherProceedings of the 1999 Optical Diagnostics of Living cells
CitySan Jose, CA, USA
Period1/25/991/26/99

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Verkhusha, V. V., Tsukita, S., & Oda, H. (1999). Analysis of cytoskeleton dynamics and cell migration in Drosophila ovaries using GFP-actin and E-cadherin-GFP fusion molecules. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 130-139). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 3604). Society of Photo-Optical Instrumentation Engineers.