An Xist-activating antisense RNA required for X-chromosome inactivation

Mrinal K. Sarkar; Srimonta Gayen; Surinder Kumar; Emily Maclary; Emily Buttigieg; Michael Hinten; Archana Kumari; Clair Harris; Takashi Sado; Sundeep Kalantry

doi:10.1038/ncomms9564

An Xist-activating antisense RNA required for X-chromosome inactivation

Mrinal K. Sarkar, Srimonta Gayen, Surinder Kumar, Emily Maclary, Emily Buttigieg, Michael Hinten, Archana Kumari, Clair Harris, Takashi Sado, Sundeep Kalantry

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

Original language	English (US)
Article number	8564
Journal	Nature communications
Volume	6
DOIs	https://doi.org/10.1038/ncomms9564
State	Published - Oct 19 2015
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/ncomms9564

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title = "An Xist-activating antisense RNA required for X-chromosome inactivation",

abstract = "The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.",

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AU - Sarkar, Mrinal K.

AU - Gayen, Srimonta

AU - Kumar, Surinder

AU - Maclary, Emily

AU - Buttigieg, Emily

AU - Hinten, Michael

AU - Kumari, Archana

AU - Harris, Clair

AU - Sado, Takashi

AU - Kalantry, Sundeep

PY - 2015/10/19

Y1 - 2015/10/19

N2 - The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

AB - The transcriptional imbalance due to the difference in the number of X chromosomes between male and female mammals is remedied through X-chromosome inactivation, the epigenetic transcriptional silencing of one of the two X chromosomes in females. The X-linked Xist long non-coding RNA functions as an X inactivation master regulator; Xist is selectively upregulated from the prospective inactive X chromosome and is required in cis for X inactivation. Here we discover an Xist antisense long non-coding RNA, XistAR (Xist Activating RNA), which is encoded within exon 1 of the mouse Xist gene and is transcribed only from the inactive X chromosome. Selective truncation of XistAR, while sparing the overlapping Xist RNA, leads to a deficiency in Xist RNA expression in cis during the initiation of X inactivation. Thus, the Xist gene carries within its coding sequence an antisense RNA that drives Xist expression.

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An Xist-activating antisense RNA required for X-chromosome inactivation

Abstract

ASJC Scopus subject areas

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