An unusual 'morphologic' variant of BF S

Donald Douglas Raum, T. Surgenor, Z. Awdeh, D. Marcus, M. Blumenthal, E. J. Yunis, C. A. Alper

Research output: Contribution to journalArticle

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Abstract

In the course of family studies of haplotypes of the alleles of the sixth chromosome loci HLA-A, C, B, D/DR, BF, C2, C4A, C4B, and glyoxalase I, we encountered an unusual BF variant. Its mobility was similar to BF F but it appeared to have a lesser intensity after staining with Coomassie Blue, and it was demonstrated by crossed immunoelectrophoresis to be present in lower concentration. It was therefore designated BF FQL. This variant was found on the haplotype HLA-A1, B17, DR7, BF*FQL, C2*C, C4A*6, C4B*1, GLO2. All other haplotypes of this type so far identified carry the BF variant BF S. Following activation of serum samples with zymosan, BF was analyzed by both agarose electrophoresis and isoelectric focusing and immunofixation. On both treatments, serum with BF SFQL produced a Ba pattern identical to that of a sample which was BF S. The Bb pattern for F and S are similar but different from those of the rare variants BF F1 and BF S1. The Bb pattern of BF FQL was, thus, as expected, the same as BF F or BF S. Hence, we conclude that the variant is a mutant from BF S with mobility similar to BF F. The mutation seemed also to have resulted in a lower concentration of product than normal.

Original languageEnglish (US)
Pages (from-to)346-351
Number of pages6
JournalAmerican Journal of Human Genetics
Volume36
Issue number2
StatePublished - 1984
Externally publishedYes

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Haplotypes
HLA-A1 Antigen
Lactoylglutathione Lyase
HLA-C Antigens
Two-Dimensional Immunoelectrophoresis
Zymosan
HLA-A Antigens
Isoelectric Focusing
Serum
Sepharose
Electrophoresis
Chromosomes
Alleles
Staining and Labeling
Mutation
Coomassie blue
HLA-B17 antigen

ASJC Scopus subject areas

  • Genetics

Cite this

Raum, D. D., Surgenor, T., Awdeh, Z., Marcus, D., Blumenthal, M., Yunis, E. J., & Alper, C. A. (1984). An unusual 'morphologic' variant of BF S. American Journal of Human Genetics, 36(2), 346-351.

An unusual 'morphologic' variant of BF S. / Raum, Donald Douglas; Surgenor, T.; Awdeh, Z.; Marcus, D.; Blumenthal, M.; Yunis, E. J.; Alper, C. A.

In: American Journal of Human Genetics, Vol. 36, No. 2, 1984, p. 346-351.

Research output: Contribution to journalArticle

Raum, DD, Surgenor, T, Awdeh, Z, Marcus, D, Blumenthal, M, Yunis, EJ & Alper, CA 1984, 'An unusual 'morphologic' variant of BF S', American Journal of Human Genetics, vol. 36, no. 2, pp. 346-351.
Raum DD, Surgenor T, Awdeh Z, Marcus D, Blumenthal M, Yunis EJ et al. An unusual 'morphologic' variant of BF S. American Journal of Human Genetics. 1984;36(2):346-351.
Raum, Donald Douglas ; Surgenor, T. ; Awdeh, Z. ; Marcus, D. ; Blumenthal, M. ; Yunis, E. J. ; Alper, C. A. / An unusual 'morphologic' variant of BF S. In: American Journal of Human Genetics. 1984 ; Vol. 36, No. 2. pp. 346-351.
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