An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates

Hee Sup Shin, Thaddeus A. Bargiello, Brian T. Clark, F. Rob Jackson, Michael W. Young

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4-7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII fragment contains a functional copy of the gene. This transforming DNA contains a single transcription unit which gives rise to a 4.5-kb poly(A)+ RNA 5. Here we report the results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates. An unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologous to DNA in chicken, mouse and man. Cloned DNAs from the mouse and Drosophila are related by long, uninterrupted tandem repetitions of the sequence ACNGGN. At the per locus, these tandem repeats are predicted to code for poly(Thr-Gly) tracts up to 48 amino acids long. These repeated sequences are also transcribed in the mouse. Several long tracts of poIy(Thr-Gly) appear to be encoded by DNA cloned from the mouse.

Original languageEnglish (US)
Pages (from-to)445-448
Number of pages4
JournalNature
Volume317
Issue number6036
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Drosophila
Vertebrates
DNA
threonyl-glycine
Genes
Courtship
Tandem Repeat Sequences
Periodicity
Sequence Homology
Chickens
Amino Acids
Messenger RNA
Mutation

ASJC Scopus subject areas

  • General

Cite this

Shin, H. S., Bargiello, T. A., Clark, B. T., Jackson, F. R., & Young, M. W. (1985). An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates. Nature, 317(6036), 445-448. https://doi.org/10.1038/317445a0

An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates. / Shin, Hee Sup; Bargiello, Thaddeus A.; Clark, Brian T.; Jackson, F. Rob; Young, Michael W.

In: Nature, Vol. 317, No. 6036, 1985, p. 445-448.

Research output: Contribution to journalArticle

Shin, HS, Bargiello, TA, Clark, BT, Jackson, FR & Young, MW 1985, 'An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates', Nature, vol. 317, no. 6036, pp. 445-448. https://doi.org/10.1038/317445a0
Shin HS, Bargiello TA, Clark BT, Jackson FR, Young MW. An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates. Nature. 1985;317(6036):445-448. https://doi.org/10.1038/317445a0
Shin, Hee Sup ; Bargiello, Thaddeus A. ; Clark, Brian T. ; Jackson, F. Rob ; Young, Michael W. / An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates. In: Nature. 1985 ; Vol. 317, No. 6036. pp. 445-448.
@article{cc4deb113a4e4879b9bac93492e84837,
title = "An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates",
abstract = "The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4-7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII fragment contains a functional copy of the gene. This transforming DNA contains a single transcription unit which gives rise to a 4.5-kb poly(A)+ RNA 5. Here we report the results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates. An unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologous to DNA in chicken, mouse and man. Cloned DNAs from the mouse and Drosophila are related by long, uninterrupted tandem repetitions of the sequence ACNGGN. At the per locus, these tandem repeats are predicted to code for poly(Thr-Gly) tracts up to 48 amino acids long. These repeated sequences are also transcribed in the mouse. Several long tracts of poIy(Thr-Gly) appear to be encoded by DNA cloned from the mouse.",
author = "Shin, {Hee Sup} and Bargiello, {Thaddeus A.} and Clark, {Brian T.} and Jackson, {F. Rob} and Young, {Michael W.}",
year = "1985",
doi = "10.1038/317445a0",
language = "English (US)",
volume = "317",
pages = "445--448",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6036",

}

TY - JOUR

T1 - An unusual coding sequence from a Drosophila clock gene is conserved in vertebrates

AU - Shin, Hee Sup

AU - Bargiello, Thaddeus A.

AU - Clark, Brian T.

AU - Jackson, F. Rob

AU - Young, Michael W.

PY - 1985

Y1 - 1985

N2 - The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4-7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII fragment contains a functional copy of the gene. This transforming DNA contains a single transcription unit which gives rise to a 4.5-kb poly(A)+ RNA 5. Here we report the results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates. An unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologous to DNA in chicken, mouse and man. Cloned DNAs from the mouse and Drosophila are related by long, uninterrupted tandem repetitions of the sequence ACNGGN. At the per locus, these tandem repeats are predicted to code for poly(Thr-Gly) tracts up to 48 amino acids long. These repeated sequences are also transcribed in the mouse. Several long tracts of poIy(Thr-Gly) appear to be encoded by DNA cloned from the mouse.

AB - The per locus has a fundamental involvement in the expression of biological rhythms in Drosophila. Mutations at this locus can shorten, lengthen or eliminate a variety of rhythmic activities that range from circadian behaviours, exemplified by eclosion and locomotor activities1,2, to short-period behaviour such as the 55-s rhythm of courtship song3. DNA from the per locus has been cloned4-7, and we have used P-element-mediated DNA transformation to establish that a 7.1-kilobase (kb) HindIII fragment contains a functional copy of the gene. This transforming DNA contains a single transcription unit which gives rise to a 4.5-kb poly(A)+ RNA 5. Here we report the results of a search for sequences homologous to the per locus DNA in the genomic DNA of several species of vertebrates. An unusual, tandemly repeated sequence forming a portion of the 4.5-kb per transcript is homologous to DNA in chicken, mouse and man. Cloned DNAs from the mouse and Drosophila are related by long, uninterrupted tandem repetitions of the sequence ACNGGN. At the per locus, these tandem repeats are predicted to code for poly(Thr-Gly) tracts up to 48 amino acids long. These repeated sequences are also transcribed in the mouse. Several long tracts of poIy(Thr-Gly) appear to be encoded by DNA cloned from the mouse.

UR - http://www.scopus.com/inward/record.url?scp=0021995424&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021995424&partnerID=8YFLogxK

U2 - 10.1038/317445a0

DO - 10.1038/317445a0

M3 - Article

C2 - 2413365

AN - SCOPUS:0021995424

VL - 317

SP - 445

EP - 448

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6036

ER -