An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling

Yumin Liu, Tianlu Chen, Yunping Qiu, Yu Cheng, Yu Cao, Aihua Zhao, Wei Jia

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Conventional chemical derivatization of metabolites in biological specimens is time-consuming, which limits the throughput and efficiency of metabolite profiling using a gas chromatography/time-of-flight mass spectrometry (GC/TOFMS) platform. We report an ultrasonication-assisted protocol which reduces the derivatization time from hours to about 30 min and significantly enhances the derivatization efficiency prior to a GC/TOFMS analysis. The protocol was evaluated using 40 compounds representing different classes of human metabolites, and demonstrated good analytical precision and accuracy. In comparison with the conventional method, the new protocol was able to increase the intensity of most of the identified peaks (71.0%) in the GC/TOFMS chromatograms of human serum samples. The detected compounds with increased intensity include most amino acids, keto-containing organic acids, carbonyl-containing carbohydrates, and unsaturated fatty acids. We applied this protocol in a metabolomic study of human serum samples obtained from 34 patients diagnosed with hypertension and 29 age- and gender-matched healthy subjects. Metabolite markers associated with hypertension, including glucosamine, d-sorbitol, 1-stearoylglycerol, and homocysteine, were identified and validated by statistical methods and use of reference standards. Our work highlights the potential of this novel approach for the large-scale metabolite profiling of samples generated from plant, animal, and clinical and epidemiological studies.

Original languageEnglish (US)
Pages (from-to)1405-1417
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Volume400
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

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Metabolites
Gas chromatography
Gas Chromatography
Mass spectrometry
Mass Spectrometry
Hypertension
Metabolomics
Sorbitol
Glucosamine
Homocysteine
Serum
Unsaturated Fatty Acids
Epidemiologic Studies
Healthy Volunteers
Carbohydrates
Organic acids
Amino Acids
Acids
Statistical methods
Animals

Keywords

  • Derivatization
  • Hypertension
  • Metabolites
  • Metabolomics
  • Ultrasonication

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling. / Liu, Yumin; Chen, Tianlu; Qiu, Yunping; Cheng, Yu; Cao, Yu; Zhao, Aihua; Jia, Wei.

In: Analytical and Bioanalytical Chemistry, Vol. 400, No. 5, 05.2011, p. 1405-1417.

Research output: Contribution to journalArticle

Liu, Yumin ; Chen, Tianlu ; Qiu, Yunping ; Cheng, Yu ; Cao, Yu ; Zhao, Aihua ; Jia, Wei. / An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 400, No. 5. pp. 1405-1417.
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