An RNA biosensor for imaging the first round of translation from single cells to living animals

James M. Halstead, Timothée Lionnet, Johannes H. Wilbertz, Frank Wippich, Anne Ephrussi, Robert H. Singer, Jeffrey A. Chao

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

Analysis of single molecules in living cells has provided quantitative insights into the kinetics of fundamental biological processes; however, the dynamics of messenger RNA (mRNA) translation have yet to be addressed. We have developed a fluorescence microscopy technique that reports on the first translation events of individual mRNA molecules. This allowed us to examine the spatiotemporal regulation of translation during normal growth and stress and during Drosophila oocyte development. We have shown that mRNAs are not translated in the nucleus but translate within minutes after export, that sequestration within P-bodies regulates translation, and that oskar mRNA is not translated until it reaches the posterior pole of the oocyte. This methodology provides a framework for studying initiation of protein synthesis on single mRNAs in living cells.

Original languageEnglish (US)
Pages (from-to)1367-1370
Number of pages4
JournalScience
Volume347
Issue number6228
DOIs
StatePublished - Mar 20 2015

ASJC Scopus subject areas

  • General

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    Halstead, J. M., Lionnet, T., Wilbertz, J. H., Wippich, F., Ephrussi, A., Singer, R. H., & Chao, J. A. (2015). An RNA biosensor for imaging the first round of translation from single cells to living animals. Science, 347(6228), 1367-1370. https://doi.org/10.1126/science.aaa3380