An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown; Paige K. Besch; Veasy C. Buttram; Norman S. Fleischer; Dean S. Skelley

An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown, Paige K. Besch, Veasy C. Buttram, Norman S. Fleischer, Dean S. Skelley

Research output: Contribution to journal › Article › peer-review

Abstract

A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

Original language	English (US)
Pages (from-to)	129-133
Number of pages	5
Journal	The Journal of Laboratory and Clinical Medicine
Volume	84
Issue number	1
State	Published - Jul 1974
Externally published	Yes

ASJC Scopus subject areas

Pathology and Forensic Medicine

Cite this

@article{8c7e899315d74aa0a3a7aafd7e37016f,

title = "An overnight radioimmunoassay for human luteinizing hormone",

abstract = "A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.",

author = "Brown, {Loretta P.} and Besch, {Paige K.} and Buttram, {Veasy C.} and Fleischer, {Norman S.} and Skelley, {Dean S.}",

year = "1974",

month = jul,

language = "English (US)",

volume = "84",

pages = "129--133",

journal = "The Journal of Laboratory and Clinical Medicine",

issn = "0022-2143",

publisher = "Mosby Inc.",

number = "1",

}

TY - JOUR

T1 - An overnight radioimmunoassay for human luteinizing hormone

AU - Brown, Loretta P.

AU - Besch, Paige K.

AU - Buttram, Veasy C.

AU - Fleischer, Norman S.

AU - Skelley, Dean S.

PY - 1974/7

Y1 - 1974/7

N2 - A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

AB - A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

UR - http://www.scopus.com/inward/record.url?scp=0016160689&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016160689&partnerID=8YFLogxK

M3 - Article

C2 - 4858004

AN - SCOPUS:0016160689

SN - 0022-2143

VL - 84

SP - 129

EP - 133

JO - The Journal of Laboratory and Clinical Medicine

JF - The Journal of Laboratory and Clinical Medicine

IS - 1

ER -

An overnight radioimmunoassay for human luteinizing hormone

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this